antibody

Goat anti-MLL2 Antibody

MBS421388

0.1 mg

290 USD

polyclonal

goat

nonconjugated

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-MLL2 Antibody

MLL2

MLL2; myeloid/lymphoid or mixed-lineage leukemia 2; ALR; ALL1-related gene; AAD10; CAGL114; KMT2B; MLL4; TNRC21; ALL1-related; trinucleotide repeat containing 21; MLL2; myeloid/lymphoid or mixed-lineage leukemia 2; ALR; ALL1-related gene; AAD10; CAGL114; KMT2B; MLL4; TNRC21; ALL1-related; trinucleotide repeat containing 21

histone-lysine N-methyltransferase 2D; Histone-lysine N-methyltransferase 2D; histone-lysine N-methyltransferase 2D; ALL1-related protein; Kabuki make-up syndrome; Kabuki mental retardation syndrome; trinucleotide repeat containing 21; histone-lysine N-methyltransferase MLL2; myeloid/lymphoid or mixed-lineage leukemia 2; lysine (K)-specific methyltransferase 2D; ALL1-related protein; Myeloid/lymphoid or mixed-lineage leukemia protein 2

MLL2

KMT2D; KMT2D; ALR; KMS; MLL2; MLL4; AAD10; KABUK1; TNRC21; CAGL114; ALR; MLL2; MLL4; Lysine N-methyltransferase 2D

KMT2D_HUMAN

Polyclonal

Goat

Human

5537

DSQNLAGEDKDSQ

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Immunohistochemistry: In paraffin embedded Human Placenta shows nuclear stainng in the trophoblasts. Similar nuclear patches are observed in Human Liver. Recommended concentration, 4-8ug/ml. Western Blot: Preliminary experiments gave bands at approx 75kDa and 25kDa in lysates of cell line K562 after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 593kDa according to NP_003473.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Peptide ELISA: antibody detection limit dilution 1:16000.

Epitope: N Terminus. Immunogen: Peptide with sequence DSQNLAGEDKDSQ-C, from the N Terminus of the protein sequence according to NP_003473.3. Corresponding Immunizing Peptide: Catalog #MBS425257

Important Note: The immunizing peptide was designed from NP_003473.1. Since then the sequence has changed in the databases and currently differs at two locations in NP_003473.3.

148762969

NP_003473.3

NM_003482.3

593,677 Da

Disease Pathway 530764!!Lysine Degradation Pathway 82956!!Lysine Degradation Pathway 320!!RNF Mutants Show Enhanced WNT Signaling And Proliferation Pathway 1016209!!Signal Transduction Pathway 477114!!Signaling By WNT In Cancer Pathway 1016191!!Signaling By Wnt Pathway 106510!!TCF Dependent Signaling In Response To WNT Pathway 980475!!XAV939 Inhibits Tankyrase, Stabilizing AXIN Pathway 1016210!!Deactivation Of The Beta-catenin Transactivating Complex Pathway 980480

The protein encoded by this gene is a histone methyltransferase that methylates the Lys-4 position of histone H3. The encoded protein is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the beta-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome. [provided by RefSeq, Oct 2010]

0.1 mg

290 USD