antibody

Goat anti-GAPDH (Internal) Antibody

MBS421359

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-GAPDH (Internal) Antibody

GAPDH

Goat Anti-GAPDH (Internal), Biotinylated Antibody; GAPDH; glyceraldehyde-3-phosphate dehydrogenase; HGNC: 4141; G3PD; GAPD; MGC88685; OTTHUMP00000174431; OTTHUMP00000174432; aging-associated gene 9 protein; glyceraldehyde 3-phosphate dehydrogenase; GAPDH antibody; glyceraldehyde-3-phosphate dehydrogenase antibody; HGNC: 4141 antibody; G3PD antibody; GAPD antibody; MGC88685 antibody; OTTHUMP00000174431 antibody; OTTHUMP00000174432 antibody; aging-associated gene 9 protein antibody; glyceraldehyde 3-phosphate dehydrogenase antibody; GAPDH (Internal)

glyceraldehyde-3-phosphate dehydrogenase isoform 1; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)

GAPDH

GAPDH; GAPDH; G3PD; GAPD; HEL-S-162eP; GAPD; GAPDH

G3P_HUMAN

Polyclonal

Goat

Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog

335

GVNHEKYDNSLK

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: In paraffin embedded Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration, 2-4ug/ml. Frozen section of Rat Liver shows textured staining of cytoplasm in hepatocytes. Recommended concentration, 10-20ug/ml. Western Blot: Approx 36kDa band observed in Human Liver, Testis and Tonsil lysates and in Mouse Spleen lysates (calculated MW of 36.1kDa according to NP_002037.2). An approx.37kDa band observed in Rat Liver lysates (calculated MW of 35.8kDa according to NP_058704.1). Recommended concentration: 0.1-0.3ug/ml.

Immunogen: Peptide with sequence C-GVNHEKYDNSLK, from the internal region of the protein sequence according to NP_002037.2; NP_001243728.1. Epitope: Internal region

Note: This antibody is expected to recognize both reported isoforms (NP_002037.2; NP_001243728.1). Reported variants represent identical protein: NP_001276674.1, NP_002037.2, NP_001276675.1. GAPDH is constitutively expressed in almost all tissues at high levels. It is therefore a useful marker when a loading/positive control is required in western blotting.

Chakir K, Daya SK, Aiba T, Tunin RS, Dimaano VL, Abraham TP, Jaques-Robinson KM, Lai EW, Pacak K, Zhu WZ, Xiao RP, Tomaselli GF, Kass DA.; Mechanisms of enhanced beta-adrenergic reserve from cardiac resynchronization therapy.; Circulation.2009 Mar 10;119(9): 1231-40.; Pubmed 19237665 . * The goat polyclonal antibody used (in WB on Human lymphocytes (PBMC)) as described in this paper was manufactured by us: ; Schillace RV, Miller CL, Pisenti N, Grotzke JE, Swarbrick GM, Lewinsohn DM, Carr DW.; A-kinase anchoring in dendritic cells is required for antigen presentation.; PLoS One.2009;4(3): e4807.; Pubmed 19277197

7669492

NP_002037.2

NM_002046.5

31,548 Da

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Gluconeogenesis Pathway (1269961); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (413342); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196)

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma. Protein type: Oxidoreductase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.2.1.12. Chromosomal Location of Human Ortholog: 12p13. Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; lipid particle; membrane; microtubule cytoskeleton; nuclear membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; ribonucleoprotein complex; vesicle. Molecular Function: glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; identical protein binding; microtubule binding; NAD binding; NADP binding; protein binding. Biological Process: gluconeogenesis; microtubule cytoskeleton organization and biogenesis; negative regulation of translation; neuron apoptosis; protein stabilization; regulation of macroautophagy

0.1 mg

300 USD