catalog number :
MBS421353
products full name :
Goat anti-SMAD4 / MADH4 Antibody
products short name :
SMAD4/MADH4
products name syn :
MADH4; SMAD4; JIP; DPC4; MAD; mothers against decapentaplegic homolog 4 (Drosophila); Mothers against decapentaplegic; Drosophila; homolog of; 4; MAD (mothers against decapentaplegic; Drosophila) homolog 4; SMAD family member 4; OTTHUMP00000163548; SMAD; mothers against DPP homolog 4; deleted in pancreatic carcinoma locus 4; mothers against decapentaplegic homolog 4; MADH4; SMAD4; JIP; DPC4; MAD, mothers against decapentaplegic homolog 4 (Drosophila); Mothers against decapentaplegic, Drosophila, homolog of, 4; MAD (mothers against decapentaplegic, Drosophila) homolog 4; SMAD family member 4; OTTHUMP00000163548; SMAD, mothers against DPP homolog 4; deleted in pancreatic carcinoma locus 4; mothers against decapentaplegic homolog 4
other names :
mothers against decapentaplegic homolog 4; Mothers against decapentaplegic homolog 4; mothers against decapentaplegic homolog 4; MAD homolog 4; SMAD, mothers against DPP homolog 4; deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; mothers against decapentaplegic, Drosophila, homolog of, 4; SMAD family member 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4
products gene name :
SMAD4
other gene names :
SMAD4; SMAD4; JIP; DPC4; MADH4; MYHRS; DPC4; MADH4; MAD homolog 4; Mothers against DPP homolog 4; SMAD 4; Smad4; hSMAD4
uniprot entry name :
SMAD4_HUMAN
reactivity :
Human, Mouse, Rat, Dog, Pig Cow
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
app notes :
Western Blot: Approx 60-65kDa band seen in mouse NIH-3T3 cell lysate. Recommended for use at 0.5-2ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000. Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from anonymous customer).
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence C-HTMPIADPQPLD, from the C Terminus of the protein sequence according to NP_005350.1.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS425590
ncbi acc num :
NP_005350.1
ncbi gb acc num :
NM_005359.5
ncbi mol weight :
60,439 Da
ncbi pathways :
ALK1 Signaling Events Pathway 137968!!ALK2 Signaling Events Pathway 137983!!Adherens Junction Pathway 83070!!Adherens Junction Pathway 481!!Androgen Receptor Signaling Pathway 198806!!BMP Signalling Pathway 545370!!BMP Receptor Signaling Pathway 137948!!BMP Signalling And Regulation Pathway 198910!!Cell Cycle Pathway 198811!!Cell Cycle Pathway 83054
ncbi summary :
This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Oct 2009]