antibody

Goat anti-Osteopontin / SPP1 Antibody

MBS421341

0.1 mg

290 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog

western blot, enzyme immunoassay

Antibody

Goat anti-Osteopontin / SPP1 Antibody

Osteopontin/SPP1

SPP1; HGNC:11255; secreted phosphoprotein 1 (osteopontin; bone sialoprotein I; early T-lymphocyte activation 1); BNSP; BSPI; ETA-1; MGC110940; OPN; Secreted phosphoprotein-1 (osteopontin; bone sialoprotein); secreted phosphoprotein 1; SPP1/CALPHA1 fusion; bone sialoprotein I; early T-lymphocyte activation 1; osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; SPP1; HGNC:11255; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); BNSP; BSPI; ETA-1; MGC110940; OPN; Secreted phosphoprotein-1 (osteopontin, bone sialoprotein); secreted phosphoprotein 1; SPP1/CALPHA1 fusion; bone sialoprotein I; early T-lymphocyte activation 1; osteopontin; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein

osteopontin isoform OPN-a; Osteopontin; osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); secreted phosphoprotein 1; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin

SPP1

SPP1; SPP1; OPN; BNSP; BSPI; ETA-1; BNSP; OPN; SPP-1

OSTP_HUMAN

Polyclonal

Goat

Human, Mouse, Rat, Dog

314

DPKSKEEDKHLK

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Western Blot: Approx 60kDa band observed in lysates of Human Tonsil and Spleen and lysates of cell line MOLT4 and Jurkat (calculated MW of 33.8kDa according to NP_000573.1). The observed molecular weight corresponds to earlier findings in literature (Singh et al, J Biol Chem. 1995 Nov 24;270(47):28471-8; PMID: 7499354). Recommended concentration: 0.5-1.5ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000.

Epitope: C Terminus. Immunogen: Peptide with sequence C-DPKSKEEDKHLK, from the C Terminus of the protein sequence according to NP_001035147.1; NP_000573.1; NP_001035149.1.

Corresponding Immunizing Peptide: Catalog #MBS425969

91206462

NP_001035147.1

NM_001040058.1

33,843 Da

BDNF Signaling Pathway 712093!!Degradation Of The Extracellular Matrix Pathway 576263!!Direct P53 Effectors Pathway 137939!!ECM-receptor Interaction Pathway 83068!!ECM-receptor Interaction Pathway 479!!Endochondral Ossification Pathway 198812!!Extracellular Matrix Organization Pathway 576262!!FGF Signaling Pathway 137989!!Focal Adhesion Pathway 198795!!Focal Adhesion Pathway 83067

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

0.1 mg

290 USD