antibody

Goat anti-AKR1C4 Antibody

MBS421331

0.1 mg

300 USD

polyclonal

goat

nonconjugated

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-AKR1C4 Antibody

AKR1C4

AKR1C4; aldo-keto reductase family 1, member C4; HGNC: 387; 3-alpha-HSD; C11; CDR; CHDR; DD4; HAKRA; MGC22581; 3-alpha hydroxysteroid dehydrogenase, type I; 3 alpha-hydroxysteroid dehydrogenase/dihydrodiol dehydrogenase 4; 3-alpha-hydroxysteroid dehydroge; AKR1C4 antibody; aldo-keto reductase family 1; member C4 antibody; HGNC: 387 antibody; 3-alpha-HSD antibody; C11 antibody; CDR antibody; CHDR antibody; DD4 antibody; HAKRA antibody; MGC22581 antibody; 3-alpha hydroxysteroid dehydrogenase; type I antibody; 3 alpha-hydroxysteroid dehydrogenase/dihydrodiol dehydrogenase 4 antibody; 3-alpha-hydroxysteroid dehydrogenase variant antibody; aldo-keto reductase family 1; member C4 antibody; chlordecone reductase antibody; dihydrodiol dehydrogenase 4 antibody; dihydrodiol dehydrogenase isozyme DD4 antibody; type I 3-alpha-hydroxysteroid dehydrogenase antibody

aldo-keto reductase family 1 member C4; Aldo-keto reductase family 1 member C4; aldo-keto reductase family 1 member C4; aldo-keto reductase family 1, member C4; 3-alpha-HSD1; 3-alpha-hydroxysteroid dehydrogenase type I (EC:1.1.1.357); Chlordecone reductase (EC:1.1.1.225); CDR; Dihydrodiol dehydrogenase 4; DD-4; DD4; HAKRA

AKR1C4

AKR1C4; AKR1C4; C11; CDR; DD4; CHDR; DD-4; HAKRA; 3-alpha-HSD; CHDR; CDR; DD-4; DD4

AK1C4_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human

323

DPKYQRVELNDGH

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 4000. Immunohistochemistry: In paraffin embedded Human Liver shows staining of distinct structures in the cytoplasm of hepatocytes. Recommended concentration, 2-4ug/ml. Western Blot: Approx.35kDa band observed in Human Liver lysates (calculated MW of 37.1kDa according to NP_001809.2). Recommended concentration: 0.1-0.3ug/ml.

Immunogen: Peptide with sequence DPKYQRVELNDGH-C, from the N Terminus of the protein sequence according to NP_001809.2. Epitope: N Terminus

Note: This antibody may cross-react with AKR1C1

24497585

NP_001809.2

NM_001818.2

37,067 Da

Benzo(a)pyrene Metabolism Pathway (198911); Bile Acid And Bile Salt Metabolism Pathway (1270040); Bile Acid Biosynthesis, Cholesterol = Cholate/chenodeoxycholate Pathway (413432); Bile Acid Biosynthesis, Cholesterol = Cholate/chenodeoxycholate Pathway (468297); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Fat-soluble Vitamins Pathway (1339147); Metabolism Of Lipids And Lipoproteins Pathway (1270001); Metabolism Of Vitamins And Cofactors Pathway (1270144); Primary Bile Acid Biosynthesis Pathway (82938)

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]

AKR1C4: Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha- androstan-3-alpha,17-beta-diol (3-alpha-diol). Also has some 20- alpha-hydroxysteroid dehydrogenase activity. The biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leads to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route. Defects in AKR1C4 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. AKR1C4 mutations may act as modifier of disease severity in SRXY8 patients. A splicing mutation resulting in loss of exon 2 has been found in affected individuals also carrying mutation Val-79 in AKR1C2 (PubMed:21802064). Belongs to the aldo/keto reductase family. Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Oxidoreductase; EC 1.1.1.357; Lipid Metabolism - androgen and estrogen; Lipid Metabolism - C21-steroid hormone; Lipid Metabolism - primary bile acid biosynthesis; EC 1.1.1.225. Chromosomal Location of Human Ortholog: 10p15.1. Cellular Component: cytoplasm; cytosol. Molecular Function: 3(or 17)-alpha-hydroxysteroid dehydrogenase activity; aldo-keto reductase activity; bile acid transmembrane transporter activity; chlordecone reductase activity; electron carrier activity; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; retinal dehydrogenase activity. Biological Process: androgen metabolic process; bile acid and bile salt transport; bile acid biosynthetic process; retinoid metabolic process; steroid metabolic process. Disease: 46,xy Sex Reversal 8

0.1 mg

300 USD