Goat anti-KPNA1/Importin alpha 5 Antibody | MyBioSource MBS421330 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-KPNA1/Importin alpha 5 Antibody

catalog :

MBS421330

quantity :

0.1 mg

price :

305 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

western blot, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

MBS421330 staining (1ug/ml) of HeLa lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

product information

catalog number :

MBS421330

products type :

Antibody

products full name :

Goat anti-KPNA1/Importin alpha 5 Antibody

products short name :

[KPNA1/Importin alpha 5]

products name syn :

[KPNA1, importin alpha 5, RCH2, SRP1, NPI-1, karyopherin alpha 1 (importin alpha 5), IPOA5, importin-alpha-S1, karyopherin alpha 1, nucleoprotein interactor 1, recombination activating gene cohort 2]

other names :

[importin subunit alpha-5; Importin subunit alpha-5; importin subunit alpha-5; karyopherin subunit alpha 1; Karyopherin subunit alpha-1; Nucleoprotein interactor 1; NPI-1]

products gene name :

[KPNA1]

other gene names :

[KPNA1; KPNA1; RCH2; SRP1; IPOA5; NPI-1; RCH2; NPI-1]

uniprot entry name :

IMA5_HUMAN

clonality :

polyclonal

host :

goat

reactivity :

Tested: Human. Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

storage stability :

Aliquot and store at -20°C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 60kDa band observed in lysates of cell lines HEK293 and HeLa (calculated MW of 60.3kDa according to NP_002255.1). Recommended concentration: 1-3ug/ml.

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: Peptide with sequence TTPGKENFRLKSY-C, from the N Terminus of the protein sequence according to NP_002255.3.

ncbi gi num :

222144293

ncbi acc num :

NP_002255.3

ncbi gb acc num :

NM_002264.3

ncbi pathways :

Activation Of DNA Fragmentation Factor Pathway (1270293); Antiviral Mechanism By IFN-stimulated Genes Pathway (1269316); Apoptosis Pathway (1270262); Apoptosis Induced DNA Fragmentation Pathway (1270292); Apoptotic Execution Phase Pathway (1270287); Class I PI3K Signaling Events Mediated By Akt Pathway (138020); Cytokine Signaling In Immune System Pathway (1269310); Disease Pathway (1268854); Early Phase Of HIV Life Cycle Pathway (1269059); HIV Infection Pathway (1269057)

ncbi summary :

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

uniprot summary :

KPNA1: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS. Belongs to the importin alpha family. Protein type: Karyopherin; Adaptor/scaffold; Nuclear import. Chromosomal Location of Human Ortholog: 3q21. Cellular Component: cytoplasm; cytosol; dendrite; nuclear pore; nucleoplasm; nucleus. Molecular Function: nuclear localization sequence binding; protein binding; protein transporter activity. Biological Process: DNA fragmentation during apoptosis; modification by virus of host cellular process; NLS-bearing substrate import into nucleus; positive regulation of protein import into nucleus; regulation of DNA recombination

size1 :

0.1 mg

price1 :

305 USD

more info or order :

MyBioSource product webpage