antibody

Goat anti-ZASP/ CYPHER/LDB3 Antibody

MBS421324

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, dog

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-ZASP/ CYPHER/LDB3 Antibody

ZASP/ CYPHER/LDB3

LDB3; ZASP; LIM domain binding 3; HGNC: 15710; KIAA01613; KIAA0613; ORACLE; PDLIM6; PDZ and LIM domain 6; Z-band alternatively spliced PDZ-motif; CYPHER; FLJ35865; ldb3z1; ldb3z4; LDB3 antibody; ZASP antibody; LIM domain binding 3 antibody; HGNC: 15710 antibody; KIAA01613 antibody; KIAA0613 antibody; ORACLE antibody; PDLIM6 antibody; PDZ and LIM domain 6 antibody; Z-band alternatively spliced PDZ-motif antibody; CYPHER antibody; FLJ35865 antibody; ldb3z1 antibody; ldb3z4 antibody; ZASP/ CYPHER / LDB3

LIM domain-binding protein 3 isoform 1; LIM domain-binding protein 3; LIM domain-binding protein 3; LIM domain binding 3; Protein cypher; Z-band alternatively spliced PDZ-motif protein

LDB3

ZASP; CYPHER

LDB3; LDB3; MFM4; ZASP; CMD1C; CMH24; CMPD3; LVNC3; CYPHER; LDB3Z1; LDB3Z4; ORACLE; PDLIM6

LDB3_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

727

KSQNKPEDEADE

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 1000. Immunohistochemistry: In paraffin embedded Human Skeletal muscle shows staining of the Z lines in the fibres. Recommended concentration, 3-6ug/ml. Data obtained from previous batch. Western Blot: Western Blot experiments gave bands at approx 90kDa and 35kDa in Human Skeletal Muscle lysate after 1ug/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Huang et al J Biol Chem.2003 Feb 28;278(9): 7360-5; PMID: 12499364). This protein has a calculated MW of 77.1kDa according to NP_009009.1. Recommended concentration: 0.1-1ug/ml.

Immunogen: Peptide with sequence C-KSQNKPEDEADE, from the internal region of the protein sequence according to NP_009009.1; NP_001073583.1; NP_001073584.1; NP_001073585.1. Epitope: Internal region

45592959

NP_009009.1

NM_007078.2

78,504 Da

This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified; all isoforms have N-terminal PDZ domains while only longer isoforms (1, 2 and 5) have C-terminal LIM domains. [provided by RefSeq, Jan 2010]

0.1 mg

300 USD