antibody

Goat anti-WISP1 Antibody

MBS421320

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat

western blot, enzyme immunoassay

Antibody

Goat anti-WISP1 Antibody

WISP1

WISP1; WNT1 inducible signaling pathway protein 1; CCN4; WISP1c; WISP1i; WISP1tc; WNT1 induced secreted protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1; WISP1 antibody; WNT1 inducible signaling pathway protein 1 antibody; CCN4 antibody; WISP1c antibody; WISP1i antibody; WISP1tc antibody; WNT1 induced secreted protein 1 antibody; Wnt-1 inducible signaling pathway protein 1 antibody; wnt-1 signaling pathway protein 1 antibody

WNT1-inducible-signaling pathway protein 1 isoform 1; WNT1-inducible-signaling pathway protein 1; WNT1-inducible-signaling pathway protein 1; WNT1 inducible signaling pathway protein 1; CCN family member 4; Wnt-1-induced secreted protein

WISP1

WISP1; WISP1; CCN4; WISP1c; WISP1i; WISP1tc; CCN4; WISP-1

WISP1_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat,

367

ESYPDFSEIAN

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: In transfected HEK293 transiently expressing WISP1 a band of approx.35kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml.

Immunogen: Peptide with sequence C-ESYPDFSEIAN, from the C Terminus of the protein sequence according to NP_003873.1; NP_543028.1. Epitope: C Terminus

Note: This antibody is expected to recognize both reported isoforms (NP_003873.1; NP_543028.1).

4507921

NP_003873.1

NM_003882.3

21,550 Da

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2011]

0.1 mg

300 USD