catalog number :
MBS421319
products full name :
Goat anti-MPG Antibody
products short name :
MPG
products name syn :
MDG; N-methylpurine-DNA glycosylase; AAG; APNG; CRA36.1; Mid1; N-methylpurine-DNA glycosylase, MPG; PIG11; PIG16; anpg; 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on 16p; CRA36.1 (3-methyl-adenine DNA glycosylase); alkylad; N-methylpurine-DNA glycosylase antibody; AAG antibody; APNG antibody; CRA36.1 antibody; MDG antibody; Mid1 antibody; N-methylpurine-DNA glycosylase; MPG antibody; PIG11 antibody; PIG16 antibody; anpg antibody; 3 1 end of the Mid1 gene; localized 68 kb upstream the humanzeta globin gene on 16p antibody; CRA36.1 (3-methyl-adenine DNA glycosylase) antibody; alkyladenine DNA glycosylase antibody; proliferation-inducing protein 11 antibody; proliferation-inducing protein 16 antibody
other names :
DNA-3-methyladenine glycosylase isoform a; DNA-3-methyladenine glycosylase; DNA-3-methyladenine glycosylase; N-methylpurine DNA glycosylase; 3-alkyladenine DNA glycosylase; 3-methyladenine DNA glycosidase; ADPG; N-methylpurine-DNA glycosylase
other gene names :
MPG; MPG; AAG; MDG; ADPG; APNG; Mid1; anpg; PIG11; PIG16; CRA36.1; AAG; ANPG; MID1
uniprot entry name :
3MG_HUMAN
reactivity :
Tested: Human; Expected from sequence similarity: Human
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Peptide ELISA: Antibody detection limit dilution 1: 1000. Western Blot: Approx 37kDa band observed in lysates of HEK293 and K562 (calculated MW of 32.9kDa according to NP_002425.2). Recommended concentration: 1-3ug/ml.
other info1 :
Immunogen: Peptide with sequence C-SVVDRVAEQDTQA, from the C Terminus of the protein sequence according to NP_002425.2; NP_001015052.1; NP_001015054.1. Epitope: C Terminus
other info2 :
Note: This antibody is expected to recognize all three reported isoforms (NP_002425.2, NP_001015052.1 and NP_001015054.1)
ncbi acc num :
NP_002425.2
ncbi gb acc num :
NM_002434.3
ncbi mol weight :
30,824 Da
ncbi pathways :
Base Excision Repair Pathway (1270351); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Base-Excision Repair, AP Site Formation Pathway (1270352); Cleavage Of The Damaged Purine Pathway (1270355); DNA Repair Pathway (1270350); Depurination Pathway (1270353); Displacement Of DNA Glycosylase By APEX1 Pathway (1270360); Recognition And Association Of DNA Glycosylase With Site Containing An Affected Purine Pathway (1270354); Resolution Of Abasic Sites (AP Sites) Pathway (1270359)
uniprot summary :
MPG: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions. Belongs to the DNA glycosylase MPG family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Nuclear receptor co-regulator; EC 3.2.2.21; Hydrolase. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: nucleoplasm. Molecular Function: alkylbase DNA N-glycosylase activity; damaged DNA binding; DNA N-glycosylase activity; DNA-3-methyladenine glycosylase I activity; protein binding. Biological Process: base-excision repair; depurination; DNA dealkylation