antibody

Goat anti-DUSP1 Antibody

MBS421313

0.1 mg

290 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog, cow

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-DUSP1 Antibody

DUSP1

DUSP1; dual specificity phosphatase 1; HVH1; CL100; MKP-1; PTPN10; serine/threonine specific protein phosphatase; MKP1; DUSP1; dual specificity phosphatase 1; HVH1; CL100; MKP-1; PTPN10; serine/threonine specific protein phosphatase; MKP1

dual specificity protein phosphatase 1; Dual specificity protein phosphatase 1; dual specificity protein phosphatase 1; MAP kinase phosphatase 1; protein-tyrosine phosphatase CL100; dual specificity protein phosphatase hVH1; serine/threonine specific protein phosphatase; mitogen-activated protein kinase phosphatase 1; dual specificity phosphatase 1; Dual specificity protein phosphatase hVH1; Mitogen-activated protein kinase phosphatase 1; MAP kinase phosphatase 1; MKP-1; Protein-tyrosine phosphatase CL100

DUSP1

DUSP1; DUSP1; HVH1; MKP1; CL100; MKP-1; PTPN10; CL100; MKP1; PTPN10; VH1; MAP kinase phosphatase 1; MKP-1

DUS1_HUMAN

Polyclonal

Goat

Human, Mouse, Rat, Dog, Cow

367

SYLQSPITTSPSC

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Immunohistochemistry: In paraffin embedded Human Prostate shows strong cytoplasm staining of secretory cells in the glands. Recommended concentration, 3-5ug/ml. Western Blot: Approx. 40kDa band observed in Human lysates of HeLa (calculated MW of 39.3kDa according to NP_004408.1). Recommended concentration: 0.3-1ug/ml. An anonymous customer found positive results in WB on Mouse microglia (BV2 cells). An anonymous customer found positive results in WB on Rat primary microglia. An anonymous customer found positive results in WB on Rat spinal cord homogenate. Peptide ELISA: antibody detection limit dilution 1:32000.

Epitope: C Terminus. Immunogen: Peptide with sequence SYLQSPITTSPSC, from the C Terminus of the protein sequence according to NP_004408.1.

Corresponding Immunizing Peptide: Catalog #MBS426138

4758204

NP_004408.1

NM_004417.3

39,298 Da

ATF-2 Transcription Factor Network Pathway 138006!!Direct P53 Effectors Pathway 137939!!EGFR1 Signaling Pathway 198782!!ErbB1 Downstream Signaling Pathway 138057!!Fc-epsilon Receptor I Signaling In Mast Cells Pathway 138050!!MAPK Signaling Pathway 198779!!MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Regulation Of P38-alpha And P38-beta Pathway 137964!!Serotonergic Synapse Pathway 525336

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq, Jul 2008]

0.1 mg

290 USD