antibody

Goat anti-APE1 / APEX1 Antibody

MBS421297

0.1 mg

290 USD

polyclonal

goat

nonconjugated

human, dog, cow

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-APE1 / APEX1 Antibody

APE1/APEX1

APE1; APEX1; APEX; APEX nuclease (multifunctional DNA repair enzyme); APE; APX; APEN; HAP1; REF1; REF-1; AP lyase; AP endonuclease class I; apurinic/apyrimidinic exonuclease; multifunctional DNA repair enzyme; DNA-(apurinic or apyrimidinic site) lyase; apurinic/apyrimidinic (abasic); APEX nuclease 1 endonuclease; deoxyribonuclease (apurinic or apyrimidinic); apurinic/apyrimidinic (abasic) endonuclease; redox factor 1; APE1; APEX; APEX nuclease (multifunctional DNA repair enzyme); APE; APX; APEN; HAP1; REF1; REF-1; AP lyase; AP endonuclease class I; apurinic/apyrimidinic exonuclease; multifunctional DNA repair enzyme; DNA-(apurinic or apyrimidinic site) lyase; apurinic/apyrimidinic (abasic); APEX nuclease 1 endonuclease; deoxyribonuclease (apurinic or apyrimidinic); apurinic/apyrimidinic (abasic) endonuclease; redox factor 1

DNA-(apurinic or apyrimidinic site) lyase; DNA-(apurinic or apyrimidinic site) lyase; DNA-(apurinic or apyrimidinic site) lyase; DNA-(apurinic or apyrimidinic site) lyase; AP lyase; protein REF-1; redox factor-1; AP endonuclease class I; apurinic-apyrimidinic endonuclease 1; apurinic/apyrimidinic (abasic) endonuclease; deoxyribonuclease (apurinic or apyrimidinic); APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease; APEN; Apurinic-apyrimidinic endonuclease 1; AP endonuclease 1; APE-1; REF-1; Redox factor-1DNA-(apurinic or apyrimidinic site) lyase, mitochondrial

APEX1

APEX1; APEX1; APE; APX; APE1; APEN; APEX; HAP1; REF1; APE; APE1; APEX; APX; HAP1; REF1; APEN; AP endonuclease 1; APE-1

APEX1_HUMAN

Polyclonal

Goat

Human, Dog, Pig, Cow

318

PKRGKKGAVAEDGD

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Immunohistochemistry: In paraffin embedded Human Breast shows nuclear staining of lobular epithelial cells. Recommended concentration, 4-6ug/ml. Western Blot: Approx 37kDa band observed in nuclear lysates of cell lines A431, HeLa and MCF7 (calculated MW of 35.6kDa according to NP_001632.2, NP_542379.1 and NP_542380.1). Recommended concentration: 0.1-0.3ug/ml. Peptide ELISA: antibody detection limit dilution 1:16000.

Epitope: N Terminus. Immunogen: Peptide with sequence PKRGKKGAVAEDGD-C, from the N Terminus of the protein sequence according to NP_001632.2. Corresponding Immunizing Peptide: Catalog #MBS425755

Important Note: Reported variants represent identical protein (NP_001632.2; NP_542379.1; NP_542380.1).

18375501

NP_001632.2

NM_001641.3

35,555 Da

BER Complex Pathway 413429!!BER Complex Pathway 468377!!Base Excision Repair Pathway 105838!!Base Excision Repair Pathway 83043!!Base Excision Repair Pathway 451!!Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway 105848!!DNA Repair Pathway 105837!!Displacement Of DNA Glycosylase By APE1 Pathway 105849!!HIF-2-alpha Transcription Factor Network Pathway 137956!!Removal Of DNA Patch Containing Abasic Residue Pathway 105851

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008]

0.1 mg

290 USD