antibody

Goat anti-Karyopherin (importin) beta 1 Antibody

MBS421247

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog, cow

western blot, enzyme immunoassay

Antibody

Goat anti-Karyopherin (importin) beta 1 Antibody

Karyopherin

KPNB1; karyopherin (importin) beta 1; HGNC: 6400; IMB1; IPOB; Impnb; MGC2155; MGC2156; MGC2157; NTF97; importin 90; importin beta-1 subunit; karyopherin beta 1; nuclear factor p97; KapB1; IPO1; karyopherin (importin) beta 1 antibody; HGNC: 6400 antibody; KPNB1 antibody; IMB1 antibody; IPOB antibody; Impnb antibody; MGC2155 antibody; MGC2156 antibody; MGC2157 antibody; NTF97 antibody; importin 90 antibody; importin beta-1 subunit antibody; karyopherin beta 1 antibody; nuclear factor p97 antibody; KapB1 antibody; IPO1 antibody; Karyopherin (importin) beta 1; Karyopherin (importin) beta 1

importin subunit beta-1 isoform 1; Importin subunit beta-1; importin subunit beta-1; karyopherin subunit beta 1; Importin-90; Karyopherin subunit beta-1; Nuclear factor p97; Pore targeting complex 97 kDa subunit; PTAC97

KPNB1

KPNB1; KPNB1; IMB1; IPO1; IPOB; Impnb; NTF97; NTF97; PTAC97

IMB1_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

876

RRSKTNKAKTLAT

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 90-100kDa band observed in lysates of cell lines Daudi, HEK293, and Jurkat (calculated MW of 97.2kDa according to NP_002256.2). Recommended concentration: 0. 03-0.1ug/ml.

Immunogen: Peptide with sequence C-RRSKTNKAKTLAT, from the C Terminus of the protein sequence according to NP_002256.2. Epitope: C Terminus

19923142

NP_002256.2

NM_002265.5

81,179 Da

Activation Of DNA Fragmentation Factor Pathway (1270293); Antiviral Mechanism By IFN-stimulated Genes Pathway (1269316); Apoptosis Pathway (1270262); Apoptosis Induced DNA Fragmentation Pathway (1270292); Apoptotic Execution Phase Pathway (1270287); Cytokine Signaling In Immune System Pathway (1269310); Disease Pathway (1268854); HIV Infection Pathway (1269057); Host Interactions Of HIV Factors Pathway (1269091); Host Interactions With Influenza Factors Pathway (1269130)

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]

KPNB1: a nuclear import protein nuclear protein import that functions either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re- exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus. Forms a complex with an importin alpha subunit. Forms a heterodimer with IPO7. Interacts with IPO7, SNUPN, RPL23A and XPO1. Two isoforms of the human protein have been reported. Belongs to the importin beta family. Protein type: Nuclear import; Karyopherin. Chromosomal Location of Human Ortholog: 17q21.32. Cellular Component: cytoplasm; cytosol; membrane; nuclear envelope; nuclear membrane; nuclear pore; nucleoplasm; protein complex. Molecular Function: enzyme binding; Hsp90 protein binding; nuclear localization sequence binding; protein binding; protein domain specific binding; protein transporter activity; Ran GTPase binding; zinc ion binding. Biological Process: astral microtubule organization and biogenesis; DNA fragmentation during apoptosis; establishment of mitotic spindle localization; establishment of protein localization; mitotic chromosome movement towards spindle pole; mitotic metaphase plate congression; modification by virus of host cellular process; NLS-bearing substrate import into nucleus; protein import into nucleus; protein import into nucleus, docking; protein import into nucleus, translocation; Ran protein signal transduction; ribosomal protein import into nucleus

0.1 mg

300 USD