antibody

Goat anti-ERG Antibody

MBS421242

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat

immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-ERG Antibody

ERG

ERG; v-ets erythroblastosis virus E26 oncogene like (avian); HGNC: 3446; erg-3; p55; transcriptional regulator ERG (transforming protein ERG); v-ets avian erythroblastosis virus E26 oncogene related; v-ets erythroblastosis virus E26 oncogene like; v-ets er; ERG antibody; v-ets erythroblastosis virus E26 oncogene like (avian) antibody; HGNC: 3446 antibody; erg-3 antibody; p55 antibody; transcriptional regulator ERG (transforming protein ERG) antibody; v-ets avian erythroblastosis virus E26 oncogene related antibody; v-ets erythroblastosis virus E26 oncogene like antibody; v-ets erythroblastosis virus E26 oncogene homolog (avian) antibody; OTTHUMP00000115970 antibody; OTTHUMP00000208036 antibody; TMPRSS2-ERG prostate cancer specific antibody; ets-related antibody

transcriptional regulator ERG isoform 1; Transcriptional regulator ERG; transcriptional regulator ERG; v-ets avian erythroblastosis virus E26 oncogene homolog; Transforming protein ERG

ERG

ERG; ERG; p55; erg-3

ERG_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat

479

PNTRLPTSHMPSH

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Prostate shows nuclear staining in secretory cells of the glands. Recommended concentration, 4-8ug/ml. Western Blot: Preliminary experiments in lysates of cell lines HEK293, A431 and Jurkat gave no specific signal but low background (at antibody concentration up to 1ug/ml).

Immunogen: Peptide with sequence C-PNTRLPTSHMPSH, from the C Terminus of the protein sequence according to NP_891548.1; NP_004440.1; NP_001129626.1; NP_001129627.1; NP_001230358.1 . Epitope: C Terminus

Note: This antibody is expected to recognise all reported isoforms (NP_891548.1; NP_004440.1; NP_001129626.1; NP_001129627.1; NP_001230358.1)

33667107

NP_891548.1

NM_182918.3

36,541 Da

Prostate Cancer Pathway (755440); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

This gene encodes a member of the erythroblast transformation-specific (ETS) family of transcriptions factors. All members of this family are key regulators of embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. The protein encoded by this gene is mainly expressed in the nucleus. It contains an ETS DNA-binding domain and a PNT (pointed) domain which is implicated in the self-association of chimeric oncoproteins. This protein is required for platelet adhesion to the subendothelium, inducing vascular cell remodeling. It also regulates hematopoesis, and the differentiation and maturation of megakaryocytic cells. This gene is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia. More than two dozens of transcript variants generated from combinatorial usage of three alternative promoters and multiple alternative splicing events have been reported, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Apr 2014]

ERG: Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure. Defects in ERG are a cause of Ewing sarcoma (ES). A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. A chromosomal aberration involving ERG is found in patients with Erwing sarcoma. Translocation t(21;22)(q22;q12) with EWSR1. Chromosomal aberrations involving ERG have been found in acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with FUS. Translocation t(X;21)(q25-26;q22) with ELF4. Belongs to the ETS family. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription factor; Oncoprotein; DNA-binding. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: cytoplasm; nucleus; ribonucleoprotein complex. Molecular Function: chromatin binding; DNA binding; protein binding; sequence-specific DNA binding; signal transducer activity. Biological Process: cell differentiation; cell migration; cell proliferation; multicellular organismal development; protein amino acid phosphorylation; regulation of transcription from RNA polymerase II promoter; signal transduction; transcription, DNA-dependent

0.1 mg

300 USD