antibody

Goat anti-CYP17A1 Antibody

MBS421228

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, dog

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-CYP17A1 Antibody

CYP17A1

CYP17A1; cytochrome P450, family 17, subfamily A, polypeptide 1; HGNC: 2593; CPT7; CYP17; P450C17; S17AH; cytochrome P450, family 17; cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia; cytochrome p450 XVIIA1; steroid 17-a; CYP17A1 antibody; cytochrome P450; family 17; subfamily A; polypeptide 1 antibody; HGNC: 2593 antibody; CPT7 antibody; CYP17 antibody; P450C17 antibody; S17AH antibody; cytochrome P450; family 17 antibody; cytochrome P450; subfamily XVII (steroid 17-alpha-hydroxylase); adrenal hyperplasia antibody; cytochrome p450 XVIIA1 antibody; steroid 17-alpha-hydroxylase/17; 20 lyase antibody; steroid 17-alpha-monooxygenase antibody

steroid 17-alpha-hydroxylase/17,20 lyase; Steroid 17-alpha-hydroxylase/17,20 lyase; steroid 17-alpha-hydroxylase/17,20 lyase; cytochrome P450 family 17 subfamily A member 1; 17-alpha-hydroxyprogesterone aldolase (EC:4.1.2.30

CYP17A1

CYP17A1; CYP17A1; CPT7; CYP17; S17AH; P450C17; CYP17; S17AH; Cytochrome P450c17

CP17A_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Cat, Dog

508

LFKDGDQKLEK

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Adenal Gland shows strong granular staining of the cytoplasm in medulla cells. Recommended concentration, 3-5ug/ml. Western Blot: Approx 60kDa band observed in human ovary lysates (calculated MW of 57.4kDa according to NP_000093.1). Recommended concentration: 0.1-0.3ug/ml.

Immunogen: Peptide with sequence C-LFKDGDQKLEK, from the internal region of the protein sequence according to NP_000093.1. Epitope: Internal region

4503195

NP_000093.1

NM_000102.3

57,371 Da

Androgen Biosynthesis Pathway (1270050); Biological Oxidations Pathway (1270189); C19/C18-Steroid Hormone Biosynthesis, Pregnenolone = Androstenedione = Estrone Pathway (413383); C19/C18-Steroid Hormone Biosynthesis, Pregnenolone = Androstenedione = Estrone Pathway (468268); C21-Steroid Hormone Biosynthesis, Progesterone = Cortisol/cortisone Pathway (413395); C21-Steroid Hormone Biosynthesis, Progesterone = Cortisol/cortisone Pathway (468370); Cytochrome P450 - Arranged By Substrate Type Pathway (1270191); Endogenous Sterols Pathway (1270192); Glucocorticoid Mineralcorticoid Metabolism Pathway (198902); Glucocorticoid Biosynthesis Pathway (1270048)

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008]

CYP17A1: Conversion of pregnenolone and progesterone to their 17- alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5). AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: salt wasting (SW, the most severe type), simple virilizing (SV, less severely affected patients), with normal aldosterone biosynthesis, non-classic form or late onset (NC or LOAH), and cryptic (asymptomatic). Belongs to the cytochrome P450 family. Protein type: Oxidoreductase; EC 4.1.2.30; EC 1.14.99.9; Lipid Metabolism - C21-steroid hormone. Chromosomal Location of Human Ortholog: 10q24.3. Cellular Component: axon; cell soma; endoplasmic reticulum; endoplasmic reticulum membrane; mitochondrion. Molecular Function: 17-alpha-hydroxyprogesterone aldolase activity; heme binding; iron ion binding; oxygen binding; steroid 17-alpha-monooxygenase activity. Biological Process: adrenal gland development; androgen biosynthetic process; biphenyl metabolic process; dibenzo-p-dioxin metabolic process; glucocorticoid biosynthetic process; hippocampus development; hormone biosynthetic process; Leydig cell differentiation; ovulation; phenol metabolic process; phthalate metabolic process; progesterone metabolic process; response to acetate; response to cAMP; response to cytokine stimulus; response to drug; response to herbicide; response to insecticide; response to ionizing radiation; response to methylmercury; response to nutrient levels; response to retinoic acid; response to steroid hormone stimulus; sex differentiation; steroid biosynthetic process; steroid metabolic process; sterol metabolic process. Disease: Adrenal Hyperplasia, Congenital, Due To 17-alpha-hydroxylase Deficiency

0.1 mg

300 USD