catalog number :
MBS421220
products full name :
Goat anti-ROBO1 / DUTT1 (Internal) Antibody
products short name :
ROBO1/DUTT1
products name syn :
ROBO1; DUTT1; roundabout; axon guidance receptor; homolog 1 (Drosophila); HGNC:10249; FLJ21882; SAX3; axon guidance receptor; roundabout 1; MGC131599; MGC133277; ROBO1; DUTT1; roundabout, axon guidance receptor, homolog 1 (Drosophila); HGNC:10249; FLJ21882; SAX3; axon guidance receptor; roundabout 1; MGC131599; MGC133277
other names :
roundabout homolog 1 isoform a; Roundabout homolog 1; roundabout homolog 1; deleted in U twenty twenty; roundabout, axon guidance receptor, homolog 1 (Drosophila); Deleted in U twenty twenty; H-Robo-1
products gene name :
ROBO1
other gene names :
ROBO1; ROBO1; SAX3; DUTT1; DUTT1
uniprot entry name :
ROBO1_HUMAN
reactivity :
Human, Mouse, Rat, Dog, Cow
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx 250kDa band observed in Human Endothelial Cell line HUVEC lysates (calculated MW of 181kDa according to NP_002932.1). Some of the observed proteolytic breakdown products are also found in Human, Mouse and Rat Brain lysates. Recommended concentration: 1-3ug/ml. Peptide ELISA: antibody detection limit dilution 1:8000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-GDVDLSNKINEMK, from the internal region of the protein sequence according to NP_002932.1; NP_598334.1; NP_001139316.1; NP_001139317.1. Corresponding Immunizing Peptide: Catalog #MBS425260
other info2 :
Important Note: This antibody is expected to recognise all reported isoforms (NP_002932.1; NP_598334.1; NP_001139316.1; NP_001139317.1).
ncbi acc num :
NP_002932.1
ncbi gb acc num :
NM_002941.3
ncbi mol weight :
169,661 Da
ncbi pathways :
Activation Of Rac Pathway 119530!!Axon Guidance Pathway 83065!!Axon Guidance Pathway 476!!Axon Guidance Pathway 105688!!Developmental Biology Pathway 477129!!Inactivation Of Cdc42 And Rac Pathway 119529!!Netrin-1 Signaling Pathway 160999!!Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway 119528!!Role Of Abl In Robo-Slit Signaling Pathway 119531!!Signaling By Robo Receptor Pathway 119527
ncbi summary :
Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]