catalog number :
MBS421216
products full name :
Goat anti-PDK1 Antibody
products short name :
PDK1
products name syn :
PDK1; pyruvate dehydrogenase kinase; isoenzyme 1; HGNC:8809; mitochondrial pyruvate dehydrogenase kinase isoenzyme 1; pyruvate dehydrogenase kinase; isoenzyme 1; OTTHUMP00000205076; mitochondrial pyruvate dehydrogenase; lipoamide; kinase isoenzyme 1; PDK1; pyruvate dehydrogenase kinase, isoenzyme 1; HGNC:8809; mitochondrial pyruvate dehydrogenase kinase isoenzyme 1; pyruvate dehydrogenase kinase, isoenzyme 1; OTTHUMP00000205076; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; pyruvate dehydrogenase kinase 1;
other names :
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial isoform 2; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; PDH kinase 1; pyruvate dehydrogenase kinase, isoenzyme 1; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1; Pyruvate dehydrogenase kinase isoform 1; PDH kinase 1
products gene name :
PDK1
other gene names :
PDK1; PDK1; PDHK1; PDH kinase 1
uniprot entry name :
PDK1_HUMAN
reactivity :
Human, Mouse, Rat, Dog, Pig, Cow
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx 65-70kDa band observed in Rat Heart lysates (calculated MW of 49.2kDa according to NP_002601.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml. Peptide ELISA: antibody detection limit dilution 1:1000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-DFKDKSAEDAK, from the internal region of the protein sequence according to NP_002601.1.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS426632
ncbi acc num :
NP_002601.1
ncbi gb acc num :
NM_002610.4
ncbi mol weight :
51,623 Da
ncbi pathways :
DNA Damage Response (only ATM Dependent) Pathway 198827!!EPO Receptor Signaling Pathway 198882!!ErbB Signaling Pathway 198844!!HIF-1 Signaling Pathway 695200!!Integrated Pancreatic Cancer Pathway 711360!!Metabolism Pathway 477135!!Pyruvate Metabolism Pathway 106205!!Pyruvate Metabolism And Citric Acid (TCA) Cycle Pathway 106327!!Regulation Of Pyruvate Dehydrogenase (PDH) Complex Pathway 106109!!The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway 477137
ncbi summary :
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]