antibody

Goat anti-Ribosomal protein L22 Antibody

MBS421200

0.1 mg

290 USD

polyclonal

goat

nonconjugated

human, mouse, rat

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-Ribosomal protein L22 Antibody

Ribosomal protein L22

RPL22; ribosomal protein L22; EAP; HBP15; HBP15/L22; 60S ribosomal protein L22; EBER-associated protein; Epstein-Barr virus small RNA-associated protein; Epstein-Barr-encoded RNA-associated protein; heparin-binding protein 15; heparin-binding protein HBp15; RPL22; ribosomal protein L22; EAP; HBP15; HBP15/L22; 60S ribosomal protein L22; EBER-associated protein; Epstein-Barr virus small RNA-associated protein; Epstein-Barr-encoded RNA-associated protein; heparin-binding protein 15; heparin-binding protein HBp15

60S ribosomal protein L22 proprotein; 60S ribosomal protein L22; 60S ribosomal protein L22; EBER-associated protein; heparin-binding protein 15; heparin-binding protein HBp15; Epstein-Barr-encoded RNA-associated protein; Epstein-Barr virus small RNA-associated protein; ribosomal protein L22; EBER-associated protein; EAP; Epstein-Barr virus small RNA-associated protein; Heparin-binding protein HBp15

RPL22

RPL22; RPL22; EAP; L22; HBP15; HBP15/L22; EAP

RL22_HUMAN

Polyclonal

Goat

Human, Mouse, Rat

128

SKESYELRYFQINQ

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Immunohistochemistry: In paraffin embedded Human Brain (Cortex) shows cytoplasm staining in neuronal bodies. Recommended concentration, 3-6ug/ml. Western Blot: Preliminary experiments gave bands at approx 17-18kDa and 25kDa in Human Liver lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.8kDa according to NP_000974.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Peptide ELISA: antibody detection limit dilution 1:16000.

Epitope: C Terminus. Immunogen: Peptide with sequence C-SKESYELRYFQINQ, from the C Terminus of the protein sequence according to NP_000974.1.

Corresponding Immunizing Peptide: Catalog #MBS425371

4506613

NP_000974.1

NM_000983.3

14,787 Da

Cap-dependent Translation Initiation Pathway 105967!!Cytoplasmic Ribosomal Proteins Pathway 198853!!Disease Pathway 530764!!Eukaryotic Translation Elongation Pathway 105976!!Eukaryotic Translation Initiation Pathway 105966!!Eukaryotic Translation Termination Pathway 105978!!Formation Of A Pool Of Free 40S Subunits Pathway 105968!!GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway 105973!!Gene Expression Pathway 105937!!Influenza Infection Pathway 106067

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 60S subunit. The protein belongs to the L22E family of ribosomal proteins. Its initiating methionine residue is post-translationally removed. The protein can bind specifically to Epstein-Barr virus-encoded RNAs (EBERs) 1 and 2. The mouse protein has been shown to be capable of binding to heparin. Transcript variants utilizing alternative polyA signals exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. It was previously thought that this gene mapped to 3q26 and that it was fused to the acute myeloid leukemia 1 (AML1) gene located at 21q22 in some therapy-related myelodysplastic syndrome patients with 3;21 translocations; however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. [provided by RefSeq, Jul 2008]

0.1 mg

290 USD