Goat anti-CD14 Antibody | MyBioSource MBS421195 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-CD14 Antibody

catalog :

MBS421195

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS421195

products type :

Antibody

products full name :

Goat anti-CD14 Antibody

products short name :

CD14

products name syn :

CD14; CD14 antigen; HGNC: 1628; CD14 antibody; CD14 antigen antibody; HGNC: 1628 antibody

other names :

monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14; monocyte differentiation antigen CD14; CD14 molecule; Myeloid cell-specific leucine-rich glycoprotein; CD_antigen: CD14

products gene name :

CD14

products gene name syn :

CD14

other gene names :

CD14; CD14

uniprot entry name :

CD14_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human

sequence length :

375

sequence :

KRVDADADPRQYAD

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Spleen shows strong clustered staining in some splenocytes. Recommended concentration, 4-6ug/ml. Western Blot: Approx 55kDa band observed in Human Lung, Lymph Node and Tonsil lysates (calculated MW of 40.1kDa according to NP_000582.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun.2001 Jun;69(6): 3772-81; PMID: 11349042). Recommended concentration: 1-3ug/ml.

other info1 :

Immunogen: Peptide with sequence C-KRVDADADPRQYAD, from the internal region of the protein sequence according to NP_000582.1. Epitope: Internal region

ncbi gi num :

4557417

ncbi acc num :

NP_000582.1

ncbi gb acc num :

NM_000591.3

ncbi mol weight :

40,076 Da

ncbi pathways :

Activated TLR4 Signalling Pathway (1269236); Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway (1269222); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Apoptosis Pathway (1270262); Caspase Activation Via Extrinsic Apoptotic Signalig Pathway (1270263); Disease Pathway (1268854); Diseases Associated With The TLR Signaling Cascade Pathway (1269157); Diseases Of Immune System Pathway (1269156); Hematopoietic Cell Lineage Pathway (83078)

ncbi summary :

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]

uniprot summary :

CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. Protein type: Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 5q31.1. Cellular Component: anchored to external side of plasma membrane; endosome membrane; extracellular region; extracellular space; Golgi apparatus; lipid raft; lipopolysaccharide receptor complex; plasma membrane. Molecular Function: lipopolysaccharide binding; opsonin receptor activity; peptidoglycan receptor activity; protein binding. Biological Process: apoptosis; cell surface receptor linked signal transduction; I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; lipopolysaccharide-mediated signaling pathway; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of MyD88-independent toll-like receptor signaling pathway; phagocytosis; positive regulation of cytokine secretion; positive regulation of endocytosis; positive regulation of interferon type I production; positive regulation of interferon-gamma production; positive regulation of tumor necrosis factor production; receptor-mediated endocytosis; response to electrical stimulus; response to ethanol; response to heat; response to magnesium ion; toll-like receptor 4 signaling pathway

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage