catalog number :
MBS421193
products full name :
Goat anti-RICTOR Antibody
products short name :
RICTOR
products name syn :
Rictor; KIAA1999; AVO3; MGC39830; likely ortholog of mouse TORC2-specific protein AVO3 (S. cerevisiae); rapamycin-insensitive companion of mTOR; mAVO3; pianissimo; DKFZp686B11164; RICTOR; RPTOR independent companion of MTOR; complex 2; PIA; mAVO3; TORC2-specific protein AVO3; RICTOR; Rictor; KIAA1999; AVO3; MGC39830; likely ortholog of mouse TORC2-specific protein AVO3 (S. cerevisiae); rapamycin-insensitive companion of mTOR; mAVO3; pianissimo; DKFZp686B11164; RPTOR independent companion of MTOR, complex 2; PIA; mAVO3; TORC2-specific protein AVO3
other names :
rapamycin-insensitive companion of mTOR isoform 1; Rapamycin-insensitive companion of mTOR; rapamycin-insensitive companion of mTOR; RICTOR; pianissimo; AVO3 homolog; TORC2-specific protein AVO3; RPTOR independent companion of MTOR, complex 2; AVO3 homolog; hAVO3
products gene name :
RICTOR
other gene names :
RICTOR; RICTOR; PIA; AVO3; hAVO3; hAVO3
uniprot entry name :
RICTR_HUMAN
reactivity :
Human, Mouse, Dog
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
app notes :
Immunohistochemistry: In paraffin embedded Kidney shows strong cytoplasmic staining of epithelial cells in distal convoluted tubules. Recommended concentration, 2-3ug/ml. Western Blot: Preliminary experiments gave an approx 38kDa band in Human Lung Carcinoma A549 cell line and cervix epitheloid carcinoma cell line HeLa lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 192kDa according to NP_689969.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide. Peptide ELISA: antibody detection limit dilution 1:4000.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence C-KQPIVDTSAES, from the C Terminus of the protein sequence according to NP_689969.2.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS426928
ncbi acc num :
NP_689969.2
ncbi gb acc num :
NM_152756.4
ncbi mol weight :
195,011 Da
ncbi pathways :
Adaptive Immune System Pathway 366160!!CD28 Co-stimulation Pathway 119553!!CD28 Dependent PI3K/Akt Signaling Pathway 119554!!CXCR3-mediated Signaling Events Pathway 138011!!CXCR4-mediated Signaling Events Pathway 137910!!Class I PI3K Signaling Events Mediated By Akt Pathway 138020!!Constitutive PI3K/AKT Signaling In Cancer Pathway 685535!!Costimulation By The CD28 Family Pathway 119552!!DAP12 Interactions Pathway 685549!!DAP12 Signaling Pathway 685550
ncbi summary :
RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM, Mar 2008]