antibody

Goat anti-Androgen Receptor Antibody

MBS421183

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog, cow

western blot, immunohistochemistry, enzyme immunoassay, chromatin immunoprecipitation

Antibody

Goat anti-Androgen Receptor Antibody

Androgen Receptor

AR; androgen receptor; dihydrotestosterone receptor; KD; AIS; TFM; DHTR; SBMA; NR3C4; SMAX1; HUMARA; HYSP1; androgen receptor; dihydrotestosterone receptor; RP11-383C12.1; androgen receptor antibody; dihydrotestosterone receptor antibody; AR antibody; KD antibody; AIS antibody; TFM antibody; DHTR antibody; SBMA antibody; NR3C4 antibody; SMAX1 antibody; HUMARA antibody; HYSP1 antibody; androgen receptor antibody; dihydrotestosterone receptor antibody; RP11-383C12.1 antibody; Androgen Receptor; Androgen Receptor

androgen receptor isoform 1; Androgen receptor; androgen receptor; androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4

AR

AR; AR; KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA; DHTR; NR3C4

ANDR_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

920

EVQLGLGRVYPRPPSC

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP)

Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration, 2-3ug/ml. Western Blot: Approx 95kDa band observed in Human Brain and Human Heart lysates (calculated MW of 99kDa according to NP_000035). Recommended concentration: 0.3-2ug/ml. Chromatin Immunoprecipitation: A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using this item with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.

Immunogen: Peptide with sequence EVQLGLGRVYPRPPSC, from the N Terminus of the protein sequence according to NP_000035.2. Epitope: N Terminus

Note: This antibody is expected to recognize isoform 1 (NP_000035.2) only.

21322252

NP_000035.2

NM_000044.3

67,659 Da

Activated PKN1 Stimulates Transcription Of AR (androgen Receptor) Regulated Genes KLK2 And KLK3 Pathway (1269513); Alpha6-Beta4 Integrin Signaling Pathway (198807); Androgen Receptor Signaling Pathway (198806); Coregulation Of Androgen Receptor Activity Pathway (138085); FOXA1 Transcription Factor Network Pathway (137979); Gene Expression Pathway (1269649); Generic Transcription Pathway (1269650); IL-6 Signaling Pathway (198864); Integrated Breast Cancer Pathway (219801); Integrated Pancreatic Cancer Pathway (711360)

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

AR: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described. Protein type: DNA-binding; Nuclear receptor; Transcription factor. Chromosomal Location of Human Ortholog: Xq12. Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; protein complex. Molecular Function: androgen binding; androgen receptor activity; ATPase binding; beta-catenin binding; chromatin binding; DNA binding; enzyme binding; ligand-dependent nuclear receptor activity; protein binding; protein dimerization activity; receptor binding; transcription factor activity; transcription factor binding; zinc ion binding. Biological Process: activation of NF-kappaB transcription factor; androgen receptor signaling pathway; cell growth; cell proliferation; cell-cell signaling; intracellular receptor-mediated signaling pathway; negative regulation of cell proliferation; negative regulation of integrin biosynthetic process; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of integrin biosynthetic process; positive regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase III promoter; positive regulation of transcription, DNA-dependent; prostate gland development; protein oligomerization; sex differentiation; signal transduction; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; transport. Disease: Androgen Insensitivity Syndrome; Androgen Insensitivity, Partial; Hypospadias 1, X-linked; Prostate Cancer; Spinal And Bulbar Muscular Atrophy, X-linked 1

0.1 mg

300 USD