catalog number :
MBS421137
products full name :
Goat anti-LIPF Antibody
products short name :
LIPF
products name syn :
LIPF; lipase; gastric; GL; HGL; HLAL; MGC138477; MGC142271; gastric lipase; gastric triacylglycerol lipase; LIPF; lipase, gastric; GL; HGL; HLAL; MGC138477; MGC142271; gastric lipase; gastric triacylglycerol lipase
other names :
gastric triacylglycerol lipase isoform 2; Gastric triacylglycerol lipase; gastric triacylglycerol lipase; gastric lipase; lipase, gastric
products gene name :
LIPF
other gene names :
LIPF; LIPF; GL; HGL; HLAL; GL; Gastric lipase
uniprot entry name :
LIPG_HUMAN
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA)
app notes :
Western Blot: Preliminary experiments gave an approx 50kDa band in Human Duodenum lysates after 0.02ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.2kDa according to NP_004181.1. The 50kDa band was successfully blocked by incubation with the immunizing peptide. Peptide ELISA: antibody detection limit dilution 1:128000.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence C-NDIVSMISEDKK, from the C Terminus of the protein sequence according to NP_004181.1.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS425984
ncbi acc num :
NP_004181.1
ncbi gb acc num :
NM_004190.3
ncbi mol weight :
42,816 Da
ncbi pathways :
Acylglycerol Degradation Pathway 413388!!Acylglycerol Degradation Pathway 468272!!Fat Digestion And Absorption Pathway 194385!!Fat Digestion And Absorption Pathway 194324!!Fatty Acid Beta Oxidation Pathway 198865!!Glycerolipid Metabolism Pathway 82986!!Glycerolipid Metabolism Pathway 361!!Metabolic Pathways 132956!!Triacylglyceride Synthesis Pathway 198859
ncbi summary :
This gene encodes gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]