antibody

Goat anti-PIK3CA/P110alpha Antibody

MBS421100

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog, cow

immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-PIK3CA/P110alpha Antibody

PIK3CA/P110alpha

PIK3CA; p110-alpha; phosphoinositide-3-kinase, catalytic, alpha polypeptide; MGC142161; MGC142163; PI3K; PI3-kinase p110 subunit alpha; PtdIns-3-kinase p110; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalyti; PIK3CA antibody; p110-alpha antibody; phosphoinositide-3-kinase; catalytic; alpha polypeptide antibody; MGC142161 antibody; MGC142163 antibody; PI3K antibody; PI3-kinase p110 subunit alpha antibody; PtdIns-3-kinase p110 antibody; phosphatidylinositol 3-kinase; catalytic; 110-KD; alpha antibody; phosphatidylinositol 3-kinase; catalytic; alpha polypeptide antibody; phosphatidylinositol-4; 5-bisphosphate 3-kinase catalytic subunit; alpha isoform antibody; PIK3CA / P110alpha

phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; Serine/threonine protein kinase PIK3CA (EC:2.7.11.1

PIK3CA

P110alpha

PIK3CA; PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; PI3K-alpha; p110-alpha; PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha

PK3CA_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

1068

HQWLKDKNKGEIYD

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Brain (Cerebral Cortex) shows cytoplasm stained in the cell bodies. Recommended concentration, 2-3ug/ml. This antibody was successfully used on Human epithelial ovarian carcinoma: Abubaker et al. Mol Cancer.2009 Jul 28;8:51. (PMID: 19638206). Western Blot: Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1ug/ml).

Immunogen: Peptide with sequence C-HQWLKDKNKGEIYD, from the internal region of the protein sequence according to NP_006209.2. Epitope: Internal region

54792082

NP_006209.2

NM_006218.3

124,284 Da

3-phosphoinositide Biosynthesis Pathway (545314); 3-phosphoinositide Biosynthesis Pathway (138600); AGE-RAGE Signaling Pathway In Diabetic Complications (1319988); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Acute Myeloid Leukemia Pathway (83117); Acute Myeloid Leukemia Pathway (529); Adaptive Immune System Pathway (1269171)

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22. [provided by RefSeq, Apr 2016]

PIK3CA: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1- AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. Heterodimer of a catalytic subunit PIK3CA and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interacts with IRS1 in nuclear extracts. Interacts with RUFY3. Interacts with RASD2. Interacts with APPL1. Interacts with HRAS1 and KRAS. Interaction with HRAS1/KRAS is required for PI3K pathway signaling and cell proliferation stimulated by EGF and FGF2. Belongs to the PI3/PI4-kinase family. Protein type: Motility/polarity/chemotaxis; EC 2.7.11.1; EC 2.7.1.153; Oncoprotein; Kinase, lipid; Carbohydrate Metabolism - inositol phosphate. Chromosomal Location of Human Ortholog: 3q26.3. Cellular Component: cytosol; lamellipodium; phosphoinositide 3-kinase complex; plasma membrane. Molecular Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; insulin receptor substrate binding; kinase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity; phosphoinositide 3-kinase activity; protein binding; protein kinase activator activity; protein serine/threonine kinase activity. Biological Process: activation of protein kinase activity; angiogenesis; cardiac muscle contraction; endothelial cell migration; epidermal growth factor receptor signaling pathway; glucose metabolic process; leukocyte migration; liver development; negative regulation of neuron apoptosis; phosphatidylinositol biosynthetic process; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; platelet activation; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation; protein kinase B signaling cascade; regulation of cellular respiration; regulation of gene expression; regulation of multicellular organism growth; regulation of phosphoinositide 3-kinase cascade; T cell costimulation; T cell receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; vasculature development. Disease: Breast Cancer; Colorectal Cancer; Congenital Lipomatous Overgrowth, Vascular Malformations, And Epidermal Nevi; Cowden Syndrome 5; Gastric Cancer; Hepatocellular Carcinoma; Keratosis, Seborrheic; Lung Cancer; Megalencephaly-capillary Malformation-polymicrogyria Syndrome; Nevus, Epidermal; Ovarian Cancer

0.1 mg

300 USD