catalog number :
MBS421073
products full name :
Goat anti-SNX5 Antibody
products short name :
SNX5
products name syn :
SNX5; FLJ10931; sorting nexin 5; RP11-504H3.2; SNX5 antibody; FLJ10931 antibody; sorting nexin 5 antibody; RP11-504H3.2 antibody
other names :
sorting nexin-5 isoform a; Sorting nexin-5; sorting nexin-5; sorting nexin 5
products gene name :
SNX5
other gene names :
SNX5; SNX5
uniprot entry name :
SNX5_HUMAN
reactivity :
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
app notes :
Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Breast shows cytoplasm staining of ductal epithelial cells. Cytoplasm staining is also observed in PCT of Human Kidney and in trophoblasts of Human Placenta. Recommended concentration, 3-6ug/ml. Western Blot: approx. 48kDa band observed in lysates of Human Kidney and cell lines Jurkat and A549 lysates (calculated MW of 46.8kDa according to NP_055241.1). Recommended concentration: 1-3ug/ml.
other info1 :
Immunogen: Peptide with sequence SLLQSCIDLFKNN, from the C Terminus of the protein sequence according to NP_055241.1; NP_689413.1. Epitope: C Terminus
ncbi acc num :
NP_055241.1
ncbi gb acc num :
NM_014426.3
ncbi mol weight :
13,349 Da
ncbi pathways :
Clathrin Derived Vesicle Budding Pathway (1269881); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Golgi Associated Vesicle Biogenesis Pathway (1269882); Membrane Trafficking Pathway (1269877); Vesicle-mediated Transport Pathway (1269876); Trans-Golgi Network Vesicle Budding Pathway (1269880)
ncbi summary :
This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein functions in endosomal sorting, the phosphoinositide-signaling pathway, and macropinocytosis. This gene may play a role in the tumorigenesis of papillary thyroid carcinoma. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2013]
uniprot summary :
SNX5: May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation. Belongs to the sorting nexin family. Protein type: Vesicle. Chromosomal Location of Human Ortholog: 20p11. Cellular Component: cytoplasmic vesicle membrane; cytosol; early endosome membrane; extrinsic to internal side of plasma membrane; phagocytic cup; retromer complex; ruffle. Molecular Function: phosphoinositide binding; protein binding; protein heterodimerization activity. Biological Process: intracellular protein transport; pinocytosis; regulation of macroautophagy; retrograde transport, endosome to Golgi; vesicle organization and biogenesis