catalog number :
MBS421059
products full name :
Goat anti-FACT / SUPT16H Antibody
products short name :
FACT/SUPT16H
products name syn :
SUPT16H; FACT; suppressor of Ty 16 homolog (S. cerevisiae); HGNC:11465; CDC68; FACTP140; FLJ10857; FLJ14010; SPT16/CDC68; chromatin-specific transcription elongation factor large subunit; FLJ34357; facilitates chromatin remodeling 140 kDa subunit; SUPT16H; FACT; suppressor of Ty 16 homolog (S. cerevisiae); HGNC:11465; CDC68; FACTP140; FLJ10857; FLJ14010; SPT16/CDC68; chromatin-specific transcription elongation factor large subunit; FLJ34357; facilitates chromatin remodeling 140 kDa subunit
other names :
FACT complex subunit SPT16; FACT complex subunit SPT16; FACT complex subunit SPT16; hSPT16; FACT 140 kDa subunit; facilitates chromatin remodeling 140 kDa subunit; facilitates chromatin transcription complex subunit SPT16; chromatin-specific transcription elongation factor 140 kDa subunit; suppressor of Ty 16 homolog (S. cerevisiae); Chromatin-specific transcription elongation factor 140 kDa subunit; FACT 140 kDa subunit; FACTp140; Facilitates chromatin transcription complex subunit SPT16; hSPT16
products gene name :
SUPT16H
other gene names :
SUPT16H; SUPT16H; CDC68; FACTP140; SPT16/CDC68; FACT140; FACTP140; hSPT16
uniprot entry name :
SP16H_HUMAN
reactivity :
Human, Mouse, Pig, Cow
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx 150kDa band observed in lysates of cell line HeLa (calculated MW of 120kDa according to NP_009123.1). Recommended concentration: 0.1-0.3ug/ml.
An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. Peptide ELISA: antibody detection limit dilution 1:8000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-DLTNKEGKKPEEK, from the internal region of the protein sequence according to NP_009123.1.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS425318
ncbi acc num :
NP_009123.1
ncbi gb acc num :
NM_007192.3
ncbi mol weight :
119,914 Da
ncbi pathways :
Disease Pathway 530764!!Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway 161052!!Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway 106004!!Formation Of RNA Pol II Elongation Complex Pathway 105950!!Gene Expression Pathway 105937!!HIV Infection Pathway 105983!!HIV Life Cycle Pathway 105984!!HIV Transcription Elongation Pathway 106001!!HIV Elongation Arrest And Recovery Pathway 161051!!Late Phase Of HIV Life Cycle Pathway 105995
ncbi summary :
Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq, Feb 2009]