Goat anti-CCKBR Antibody | MyBioSource MBS421039 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-CCKBR Antibody

catalog :

MBS421039

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS421039

products type :

Antibody

products full name :

Goat anti-CCKBR Antibody

products short name :

CCKBR

products name syn :

CCKBR; cholecystokinin B receptor; HGNC: 1571; CCK-B; GASR; CCK2 receptor; cholecystokinin-B receptor/gastrin receptor; gastrin receptor; gastrin cholecystokinin brain receptor; CCK2R; CCKBR antibody; cholecystokinin B receptor antibody; HGNC: 1571 antibody; CCK-B antibody; GASR antibody; CCK2 receptor antibody; cholecystokinin-B receptor/gastrin receptor antibody; gastrin receptor antibody; gastrin cholecystokinin brain receptor antibody; CCK2R antibody

other names :

gastrin/cholecystokinin type B receptor isoform 1; Gastrin/cholecystokinin type B receptor; gastrin/cholecystokinin type B receptor; cholecystokinin B receptor; Cholecystokinin-2 receptor; CCK2-R

products gene name :

CCKBR

other gene names :

CCKBR; CCKBR; GASR; CCK-B; CCK2R; CCKRB; CCK-B receptor; CCK-BR; CCK2-R

uniprot entry name :

GASR_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human, Rat; Expected from sequence similarity: Human, Mouse, Rat, Cow

sequence length :

447

sequence :

FDGDSDSDSQSRVRNQ

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Pancreas shows staining of the islets of Langerhans. Recommended concentration, 4-6ug/ml. Western Blot: Approx.75kDa band observed in purified membranes of Rat insulinoma cell line RIN14B (calculated MW of 48.4kDa according to NP_033957.1). Data were published by El-Kouhen K and Morisset J, Int J Endocrinol.2009;2009: 875167; PMID: 20011057. The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem.2003 Nov;51(11): 1501-13; PMID: 14566022). Data obtained from antibodies from a previous goat. Recommended concentration: 0.1-0.3ug/ml. Immunocytochemsitry/Immunofluorescence: This antibody has been successfully used in ICC/IF for RIN-14B cells as described in the following paper: El-Kouhen et al, Pancreas.2010 Mar;39(2): 127-34, PMID: 19959964 and for rat pancreatic stellate cells as described in: Berna et al, J Biol Chem. 2010 Dec 10;285(50):38905-14, PMID: 20843811. Data obtained from antibodies from a previous goat.

other info1 :

Immunogen: Peptide with sequence C-FDGDSDSDSQSRVRNQ, from the internal region of the protein sequence according to NP_795344.1. Epitope: Internal region

ncbi gi num :

28875799

ncbi acc num :

NP_795344.1

ncbi gb acc num :

NM_176875.3

ncbi mol weight :

41,504 Da

ncbi pathways :

Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); G Alpha (q) Signalling Events Pathway (1269578); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); GPCRs, Class A Rhodopsin-like Pathway (198886); GPCRs, Other Pathway (198765); Gastric Acid Secretion Pathway (154409); Gastric Acid Secretion Pathway (154383)

ncbi summary :

This gene encodes a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. Alternative splicing results in multiple transcript variants. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors. [provided by RefSeq, Dec 2015]

uniprot summary :

CCKBR: Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 11p15.4. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: 1-phosphatidylinositol-3-kinase regulator activity; cholecystokinin receptor activity; gastrin receptor activity; phosphoinositide phospholipase C activity; protein binding; type B gastrin/cholecystokinin receptor binding. Biological Process: cell proliferation; cell surface receptor linked signal transduction; digestion; elevation of cytosolic calcium ion concentration; feeding behavior; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); gastric acid secretion; gland development; gut development; positive regulation of cell proliferation; regulation of phosphoinositide 3-kinase activity; sensory perception

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage