antibody

Goat anti-ALMS1 Antibody

MBS421034

0.1 mg

290 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog, cow

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-ALMS1 Antibody

ALMS1

ALMS1; Alstrom syndrome 1; ALSS; KIAA0328; DKFZp686A118; DKFZp686D1828; Alstrom syndrome 1; ALSS; KIAA0328; DKFZp686A118; DKFZp686D1828

Alstrom syndrome protein 1; Alstrom syndrome protein 1; Alstrom syndrome protein 1; Alstrom syndrome 1

ALMS1

ALMS1; ALMS1; ALSS; KIAA0328

ALMS1_HUMAN

Polyclonal

Goat

Human, Mouse, Rat, Dog, Cow

4169

RVTNQLLGRKVPWD

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Immunohistochemistry: In paraffin embedded Human Testis shows staining of cytoplasm in spermatocytes. Recommended concentration, 1-3ug/ml. Western Blot: Preliminary experiments gave bands at approx 200kDa and 75kDa in lysates of Human T cell line Jurkat after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 461kDa according to NP_055935.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Peptide ELISA: antibody detection limit dilution 1:32000.

Epitope: C Terminus. Immunogen: Peptide with sequence C-RVTNQLLGRKVPWD, from the C Terminus of the protein sequence according to NP_055935.4. Corresponding Immunizing Peptide: Catalog #MBS426329

Important Note: The C terminus of ALMS1 shares an 8 amino acid stretch with two other human proteins: hypothetical protein (XP_169104) and ERAL1 (NP_005693). However, this stretch is located internally in these two proteins and so will most likely not cross-react with this antibody. The N terminus was not chosen as the peptide is expected to cyclise during synthesis.

110349786

NP_055935.4

NM_015120.4

425,137 Da

Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!Centrosome Maturation Pathway 105807!!G2/M Transition Pathway 105801!!Loss Of Nlp From Mitotic Centrosomes Pathway 105811!!Loss Of Proteins Required For Interphase Microtubule Organization From The Centrosome Pathway 105810!!Mitotic G2-G2/M Phases Pathway 160942!!Recruitment Of Mitotic Centrosome Proteins And Complexes Pathway 105808!!Regulation Of PLK1 Activity At G2/M Transition Pathway 771569

This gene encodes a protein containing a large tandem-repeat domain as well as additional low complexity regions. The encoded protein functions in microtubule organization, particularly in the formation and maintanance of cilia. Mutations in this gene cause Alstrom syndrome. There is a pseudogene for this gene located adjacent in the same region of chromosome 2. Alternative splice variants have been described but their full length nature has not been determined. [provided by RefSeq, Apr 2014]

0.1 mg

290 USD