catalog number :
MBS420993
products full name :
Goat anti-EHD2 Antibody
products short name :
EHD2
products name syn :
EHD2; PAST2; EH-domain containing 2; EH domain containing 2; FLJ96617; EHD2; PAST2; EH-domain containing 2; EH domain containing 2; FLJ96617
other names :
EH domain-containing protein 2; EH domain-containing protein 2; EH domain-containing protein 2; PAST homolog 2; EH domain containing 2; EH-domain containing 2; PAST homolog 2
products gene name :
EHD2
other gene names :
EHD2; EHD2; PAST2; PAST2
uniprot entry name :
EHD2_HUMAN
reactivity :
Human, Mouse, Rat, Dog, Cow
sequence :
CRLVPPSKRRHKGSA
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx 65kDa band observed in Human Placenta lysates (calculated MW of 61.2kDa according to NP_055416.2). In transfected HEK293 transiently expressing EHD2 a band of approx. 62kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3ug/ml. Peptide ELISA: antibody detection limit dilution 1:64000.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence CRLVPPSKRRHKGSA, from the C Terminus of the protein sequence according to NP_055416.2. Corresponding Immunizing Peptide: Catalog #MBS426788
other info2 :
Important Note: This antibody is expected to recognise EHD1 protein as well as EHD2.
ncbi acc num :
NP_055416.2
ncbi gb acc num :
NM_014601.3
ncbi mol weight :
46,059 Da
ncbi pathways :
Endocytosis Pathway 102279!!Endocytosis Pathway 102181!!Factors Involved In Megakaryocyte Development And Platelet Production Pathway 187196!!Hemostasis Pathway 106028!!Insulin Signaling Pathway 198845
ncbi summary :
This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. [provided by RefSeq, Jul 2008]