Goat anti-PCSK9 (C Terminus) Antibody | MyBioSource MBS420991 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-PCSK9 (C Terminus) Antibody

catalog :

MBS420991

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420991

products type :

Antibody

products full name :

Goat anti-PCSK9 (C Terminus) Antibody

products short name :

PCSK9

products name syn :

Goat Anti-PCSK9 (aa214-228) Antibody; PCSK9; FH3; NARC1; HCHOLA3; proprotein convertase subtilisin/kexin type 9; neural apoptosis regulated convertase 1; hypercholesterolemia, autosomal dominant 3; proprotein convertase subtilisin /kexin type 9; LDLCQ1; NARC-1; PCSK9 antibody; FH3 antibody; NARC1 antibody; HCHOLA3 antibody; proprotein convertase subtilisin/kexin type 9 antibody; neural apoptosis regulated convertase 1 antibody; hypercholesterolemia; autosomal dominant 3 antibody; proprotein convertase subtilisin /kexin type 9 antibody; LDLCQ1 antibody; NARC-1 antibody; PCSK9 (C Terminus)

other names :

proprotein convertase subtilisin/kexin type 9 preproprotein; Proprotein convertase subtilisin/kexin type 9; proprotein convertase subtilisin/kexin type 9; proprotein convertase subtilisin/kexin type 9; Neural apoptosis-regulated convertase 1; NARC-1; Proprotein convertase 9; PC9; Subtilisin/kexin-like protease PC9

products gene name :

PCSK9

other gene names :

PCSK9; PCSK9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3; NARC1; NARC-1; PC9

uniprot entry name :

PCSK9_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human

sequence length :

692

sequence :

CRSRHLAQASQELQ

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 75kDa band observed in Human Adipose and Pancreas lysates (calculated MW of 74.3kDa according to NP_777596.2). Recommended concentration: 0.3-1µg/ml. Detects transfected human pcsk9 in McA cell lysates (see figure) as two bands of approx. 73 and 60 kDa, representing unprocessed and processed protein. Constructs and lysates were as described in Sun et al Hum Mol Genet. 2005 May 1;14(9):1161-9, PMID: 15772090. Data obtained from antibodies from a previous goat. An anonymous customer found positive results in WB on Human Liver. IHC: In paraffin embedded Human Kidney shows ER and nuclear membrane staining in PCT. Recommended concentration, 2-6ug/ml. Data obtained from antibodies from a previous goat.

other info1 :

Immunogen: Peptide with sequence CRSRHLAQASQELQ, from the C Terminus of the protein sequence according to NP_777596.2. Epitope: C Terminus

ncbi gi num :

31317307

ncbi acc num :

NP_777596.2

ncbi gb acc num :

NM_174936.3

ncbi mol weight :

20,827 Da

ncbi summary :

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

uniprot summary :

PCSK9: Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na(+) channel (ENaC)-mediated Na(+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways. Defects in PCSK9 are the cause of hypercholesterolemia autosomal dominant type 3 (HCHOLA3). A familial condition characterized by elevated circulating cholesterol contained in either low-density lipoproteins alone or also in very-low-density lipoproteins. Belongs to the peptidase S8 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: EC 3.4.21.-; Secreted, signal peptide; Secreted; Cell development/differentiation; Protease. Chromosomal Location of Human Ortholog: 1p32.3. Cellular Component: cell surface; cytoplasm; early endosome; endoplasmic reticulum; ER to Golgi transport vesicle; extracellular space; extrinsic to external side of plasma membrane; Golgi apparatus; late endosome; lysosome; perinuclear region of cytoplasm; plasma membrane; rough endoplasmic reticulum. Molecular Function: apolipoprotein binding; apolipoprotein receptor binding; low-density lipoprotein binding; low-density lipoprotein receptor binding; protein binding; protein self-association; receptor inhibitor activity; serine-type endopeptidase activity; sodium channel inhibitor activity; very-low-density lipoprotein binding. Biological Process: apoptosis; cellular response to insulin stimulus; cellular response to starvation; cholesterol homeostasis; cholesterol metabolic process; kidney development; lipoprotein metabolic process; liver development; low-density lipoprotein receptor catabolic process; lysosomal transport; negative regulation of receptor recycling; neurogenesis; neuron differentiation; phospholipid metabolic process; positive regulation of low-density lipoprotein receptor catabolic process; positive regulation of neuron apoptosis; positive regulation of receptor internalization; protein autoprocessing; regulation of neuron apoptosis; regulation of receptor activity; triacylglycerol metabolic process. Disease: Hypercholesterolemia, Autosomal Dominant, 3

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage