antibody

Goat anti-DAP3 Antibody

MBS420985

0.1 mg

290 USD

polyclonal

goat

nonconjugated

western blot, immunocytochemistry, enzyme immunoassay

Antibody

Goat anti-DAP3 Antibody

DAP3

DAP3; death associated protein 3; DAP-3; DKFZp686G12159; MGC126058; MGC126059; MRP-S29; MRPS29; bMRP-10; death-associated protein 3; mitochondrial 28S ribosomal protein S29; DAP3; death associated protein 3; DAP-3; DKFZp686G12159; MGC126058; MGC126059; MRP-S29; MRPS29; bMRP-10; death-associated protein 3; mitochondrial 28S ribosomal protein S29

28S ribosomal protein S29, mitochondrial isoform 1; 28S ribosomal protein S29, mitochondrial; 28S ribosomal protein S29, mitochondrial; S29mt; death-associated protein 3; mitochondrial 28S ribosomal protein S29; ionizing radiation resistance conferring protein; death associated protein 3; Death-associated protein 3; DAP-3; Ionizing radiation resistance conferring protein

DAP3

DAP3; DAP3; DAP-3; MRPS29; MRP-S29; bMRP-10; MRPS29; MRP-S29; S29mt; DAP-3

RT29_HUMAN

Polyclonal

Goat

Human

398

NPSLLERHCAYL

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)

Western Blot: Approx 40kDa band observed in lysates of cell line HeLa (calculated MW of 45.6kDa according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293. The band fades in HeLa after si-RNA-mediated knock-down (including the fainter shoulder band at 37kDa). Recommended concentration: 1-3ug/ml. Peptide ELISA: antibody detection limit dilution 1:16000. Immunocytochemistry: Mitochondrial staining of HeLa after in situ denaturation of the target protein using 3-4M guanidinium thiocyanate (patent pending). The subcellular localisation is in agreement with Morgan et al., Biochem Biophys Res Commun. 2001 Jan 12;280(1):177-81.(PMID: 11162496) and Harada et al., Apoptosis. 2010 Oct;15(10):1247-55.(PMID: 20563667). The signal goes down upon knockdown of the DAP3 expression.Recommended concentration: 3-10ug/ml.

Epitope: C Terminus. Immunogen: Peptide with sequence NPSLLERHCAYL, from the C Terminus of the protein sequence according to NP_387506.1; NP_001186779.1; NP_001186780.1. Corresponding Immunizing Peptide: Catalog #MBS426650

Important Note: This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1; NP_004623.1; NP_001186778.1).

16905526

NP_387506.1

NM_033657.2

41,673 Da

TRAIL Signaling Pathway 137920

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq, Dec 2010]

0.1 mg

290 USD