Goat anti-LXR alpha/LXR beta Antibody | MyBioSource MBS420979 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-LXR alpha/LXR beta Antibody

catalog :

MBS420979

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

western blot, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420979

products type :

Antibody

products full name :

Goat anti-LXR alpha/LXR beta Antibody

products short name :

LXR alpha/LXR beta

products name syn :

NR1H3; LXRA; LXR-a; RLD-1; nuclear receptor subfamily 1, group H, member 3; liver X receptor, alpha; NR1H2; NER; UNR; LXR-b; NER-I; RIP15; nuclear receptor subfamily 1, group H, member 2; ubiquitously-expressed nuclear receptor / NR1H2; NER; UNR; LXR-b; N; LXRA antibody; LXR-a antibody; RLD-1 antibody; NR1H3 antibody; nuclear receptor subfamily 1; group H; member 3 antibody; liver X receptor; alpha antibody; NR1H2 antibody; NER antibody; UNR antibody; LXR-b antibody; NER-I antibody; RIP15 antibody; nuclear receptor subfamily 1; group H; member 2 antibody; ubiquitously-expressed nuclear receptor / NR1H2 antibody; NER antibody; UNR antibody; LXR-b antibody; NER-I antibody; RIP15 antibody; nuclear receptor subfamily 1; group H; member 2 antibody; ubiquitously-expressed nuclear receptor antibody; OTTHUMP00000198038 antibody; liver X receptor alpha antibody; liver X receptor-alpha antibody; LXRB antibody; LX receptor beta antibody; iver X receptor beta antibody; liver X receptor-beta antibody; nuclear orphan receptor LXR-beta antibody; oxysterols receptor LXR-beta antibody; LXR alpha / LXR beta

other names :

oxysterols receptor LXR-alpha isoform 1; Oxysterols receptor LXR-alpha; oxysterols receptor LXR-alpha; nuclear receptor subfamily 1 group H member 3; Liver X receptor alpha; Nuclear receptor subfamily 1 group H member 3

products gene name :

NR1H3

products gene name syn :

NR1H2; LXR alpha; LXR beta

other gene names :

NR1H3; NR1H3; LXRA; LXR-a; RLD-1; LXRA

uniprot entry name :

NR1H3_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

sequence length :

447

sequence :

CRLQDKKLPPLLSEI

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 55kDa band observed in Human, Mouse and Rat spleen lysates (calculated MW of 50.5kDa according to NP_005684 and 50.1kDa according to NP_009052). Recommended concentration: 1-3ug/ml. Additional minor bands were also consistently observed in Human Spleen at 38kDa and 75kDa. These bands were successfully blocked by incubation with the immunising peptide.

other info1 :

Immunogen: Peptide with sequence CRLQDKKLPPLLSEI, from the internal region of the protein sequence according to NP_005684.2; NP_009052.3. Epitope: Internal region

other info2 :

Note: This antibody is expected to recognise an epitope corresponding to aa 429-442 of human LXR alpha protein (NP_005684.2) and to aa 443-456 of human LXR beta protein (NP_009052.3).

ncbi gi num :

194294517

ncbi acc num :

NP_005684.2

ncbi gb acc num :

NM_005693.3

ncbi mol weight :

45,692 Da

ncbi pathways :

Adipogenesis Pathway (198832); Gene Expression Pathway (1269649); Generic Transcription Pathway (1269650); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Insulin Resistance Pathway (1272486); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862188); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862314); Nuclear Receptor Transcription Pathway (1269652); Nuclear Receptors Pathway (198848)

ncbi summary :

The protein encoded by this gene belongs to the NR1 subfamily of the nuclear receptor superfamily. The NR1 family members are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. This protein is highly expressed in visceral organs, including liver, kidney and intestine. It forms a heterodimer with retinoid X receptor (RXR), and regulates expression of target genes containing retinoid response elements. Studies in mice lacking this gene suggest that it may play an important role in the regulation of cholesterol homeostasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

uniprot summary :

LXR-alpha: Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand- binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half- sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Belongs to the nuclear hormone receptor family. NR1 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Motility/polarity/chemotaxis; Nuclear receptor. Chromosomal Location of Human Ortholog: 11p11.2. Cellular Component: nuclear chromatin; nucleoplasm; nucleus; receptor complex. Molecular Function: cholesterol binding; DNA binding; ligand-dependent nuclear receptor activity; protein binding; sequence-specific DNA binding; steroid hormone receptor activity; sterol response element binding; transcription coactivator activity; zinc ion binding. Biological Process: apoptotic cell clearance; cholesterol homeostasis; intracellular receptor-mediated signaling pathway; lipid homeostasis; negative regulation of inflammatory response; negative regulation of lipid transport; negative regulation of macrophage activation; negative regulation of pinocytosis; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cellular protein metabolic process; positive regulation of cholesterol transport; positive regulation of fatty acid biosynthetic process; positive regulation of lipoprotein lipase activity; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of circadian rhythm; response to progesterone stimulus; steroid hormone mediated signaling; sterol homeostasis; transcription initiation from RNA polymerase II promoter

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage