Goat anti-ITGAM/CD11B Antibody | MyBioSource MBS420973 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-ITGAM/CD11B Antibody

catalog :

MBS420973

quantity :

0.1 mg

price :

305 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Immunofluorescence analysis of paraformaldehyde fixed U937 cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by AlexaFluor 488 secondary antibody (4ug/ml)

image 2 :

(2ug/ml) staining of paraffin embedded Human Brain. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

product information

catalog number :

MBS420973

products type :

Antibody

products full name :

Goat anti-ITGAM/CD11B Antibody

products short name :

[ITGAM/CD11B]

products name syn :

[ITGAM; integrin, alpha M (complement component 3 receptor 3 subunit); CD11B; CR3A; MAC-1; MAC1A; MGC117044; MO1A; Integrin, alpha-M (complement component receptor-3, alpha); antigen CD11B (p170); macrophage antigen, alpha polypeptide; antigen CD11b (p; ITGAM antibody; integrin; alpha M (complement component 3 receptor 3 subunit) antibody; CD11B antibody; CR3A antibody; MAC-1 antibody; MAC1A antibody; MGC117044 antibody; MO1A antibody; Integrin; alpha-M (complement component receptor-3; alpha) antibody; antigen CD11B (p170) antibody; macrophage antigen; alpha polypeptide antibody; antigen CD11b (p170) antibody; complement component receptor 3; alpha antibody; integrin alpha M antibody; integrin; alpha M (complement component receptor 3; alpha) antibody; CD11b (p170); macrophage antigen alpha polypeptide antibody; macrophage antigen alpha polypeptide antibody; neutrophil adherence receptor alpha-M subunit antibody; ITGAM / CD11B]

other names :

[integrin alpha-M isoform 2; Integrin alpha-M; integrin alpha-M; integrin subunit alpha M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD_antigen: CD11b]

products gene name :

[ITGAM]

products gene name syn :

[CD11B]

other gene names :

[ITGAM; ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6; CD11B; CR3A]

uniprot entry name :

ITAM_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human. Expected from sequence similarity: Human

sequence length :

1152

sequence :

RNDGEDSYRTQ

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

storage stability :

Aliquot and store at -20°C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Peptide ELISA: antibody detection limit dilution 1:32000. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U937 cells. Recommended concentration: 10 ug/ml.

image1 heading :

Immunofluorescence (IF)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: Peptide with sequence C-RNDGEDSYRTQ, from the internal region of the protein sequence according to NP_000623.2. Epitope: Internal region

other info2 :

Note: The immunizing peptide represents part of the extra-cellular domain.

ncbi gi num :

88501734

ncbi acc num :

NP_000623.2

ncbi gb acc num :

NM_000632.3

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (1269373); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Extracellular Matrix Organization Pathway (1270244); Focal Adhesion Pathway (198795); Hematopoietic Cell Lineage Pathway (83078)

ncbi summary :

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

uniprot summary :

ITGAM: a single-pass type I membrane protein of the integrin alpha chain family. Implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Also known as CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. A receptor for fibrinogen, factor X and ICAM1. Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Predominantly expressed in monocytes and granulocytes. Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 16p11.2. Cellular Component: cell surface; extracellular space; plasma membrane. Molecular Function: glycoprotein binding; protein binding. Biological Process: extracellular matrix organization and biogenesis; leukocyte migration; toll-like receptor 4 signaling pathway. Disease: Systemic Lupus Erythematosus, Susceptibility To, 6

size1 :

0.1 mg

price1 :

305 USD

more info or order :

MyBioSource product webpage