catalog number :
MBS420948
products full name :
Goat anti-PDE5A Antibody
products short name :
PDE5A
products name syn :
PDE5A; CN5A; PDE5; PDE5A1; CGB-PDE; phosphodiesterase 5A; cGMP-specific; cGMP-binding cGMP-specific 3 1; 5 1-cyclic nucleotide phosphodiesterase; PDE5A; CN5A; PDE5; PDE5A1; CGB-PDE; phosphodiesterase 5A, cGMP-specific; cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase
other names :
cGMP-specific 3',5'-cyclic phosphodiesterase isoform 1; cGMP-specific 3',5'-cyclic phosphodiesterase; cGMP-specific 3',5'-cyclic phosphodiesterase; phosphodiesterase isozyme 5; cGMP-specific phosphodiesterase PDE5A2; cGMP-specific phosphodiesterase type 5A; cGMP-binding cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase; phosphodiesterase 5A, cGMP-specific; cGMP-binding cGMP-specific phosphodiesterase; CGB-PDE
products gene name :
PDE5A
other gene names :
PDE5A; PDE5A; CN5A; PDE5; CGB-PDE; PDE5; CGB-PDE
uniprot entry name :
PDE5A_HUMAN
reactivity :
Human, Rat, Dog, Cow
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx 100kDa band observed in Human Lung lysates (calculated MW of 100kDa according to isoform 1, NP_001074.2). In transfected HEK293 transiently expressing PDE5A isoform 2 a band of approx. 95kDa is observed. This band is not observed in the non-transfected HEK293 (calculated MW of 94.8kDa according to isoform 2, NP_236914.2). Recommended concentration: 0.3-1ug/ml. Peptide ELISA: antibody detection limit dilution 1:16000.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence C-LINGESGQAKRN, from the C Terminus of the protein sequence according to NP_001074.2; NP_246273.2; NP_236914.2; (NP_237223). Corresponding Immunizing Peptide: Catalog #MBS426075
other info2 :
Important Note: This antibody is expected to recognise reported isoforms NP_001074.2, NP_236914.2 and NP_246273.2.
ncbi acc num :
NP_001074.2
ncbi gb acc num :
NM_001083.3
ncbi mol weight :
94,805 Da
ncbi pathways :
Hemostasis Pathway 106028!!Nitric Oxide Stimulates Guanylate Cyclase Pathway 187200!!Platelet Homeostasis Pathway 187198!!Purine Metabolism Pathway 82944!!Purine Metabolism Pathway 307!!CGMP Effects Pathway 187201!!CGMP-PKG Signaling Pathway 983748!!CGMP-PKG Signaling Pathway 985584
ncbi summary :
This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]