catalog number :
MBS420935
products full name :
Goat anti-VPS41 Antibody
products short name :
VPS41
products name syn :
VPS41; vacuolar protein sorting 41 (yeast); HGNC:12713; HVSP41; vacuolar assembly protein 41; vacuolar protein sorting 41 (yeast homolog); vacuolar protein sorting 41 homolog (S. cerevisiae); HVPS41; hVps41p; vacuolar protein sorting 41; VPS41; vacuolar protein sorting 41 (yeast); HGNC:12713; HVSP41; vacuolar assembly protein 41; vacuolar protein sorting 41 (yeast homolog); vacuolar protein sorting 41 homolog (S. cerevisiae); HVPS41; hVps41p; vacuolar protein sorting 41
other names :
vacuolar protein sorting-associated protein 41 homolog isoform 1; Vacuolar protein sorting-associated protein 41 homolog; vacuolar protein sorting-associated protein 41 homolog; S53; vacuolar assembly protein 41; vacuolar protein sorting 41 homolog (S. cerevisiae); S53
products gene name :
VPS41
other gene names :
VPS41; VPS41; HVPS41; HVSP41; hVps41p
uniprot entry name :
VPS41_HUMAN
reactivity :
Human, Mouse, Rat, Dog, Cow
sequence :
DHLKKDSQNKTLLK
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
app notes :
Immunohistochemistry: In paraffin embedded Human Kidney shows staining of collective tubules. Recommended concentration, 4-6ug/ml. Western Blot: Preliminary experiments gave bands at approx 48kDa and 28kDa in human duodenum, kidney and placenta lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 98.5kDa according to NP_055211.1 and 100kDa according to NP_542198.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Have any further splice variants/modified forms been reported. Peptide ELISA: antibody detection limit dilution 1:16000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-DHLKKDSQNKTLLK, from the internal region of the protein sequence according to NP_055211.2; NP_542198.2. Corresponding Immunizing Peptide: Catalog #MBS426666
other info2 :
Important Note: This antibody is expected to recognise both reported isoforms (NP_055211.2 and NP_542198.2)
ncbi acc num :
NP_055211.2
ncbi gb acc num :
NM_014396.3
ncbi mol weight :
95,924 Da
ncbi summary :
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human ortholog of yeast Vps41 protein which is also conserved in Drosophila, tomato, and Arabidopsis. Expression studies in yeast and human indicate that this protein may be involved in the formation and fusion of transport vesicles from the Golgi. Several transcript variants encoding different isoforms have been described for this gene, however, the full-length nature of not all is known. [provided by RefSeq, Jul 2008]