antibody

Goat anti-AXIN1 Antibody

MBS420920

0.1 mg

290 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog, cow

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-AXIN1 Antibody

AXIN1

AXIN1; axin 1; AXIN; LA16c-314G4.3; MGC52315; axis inhibition protein 1; axis inhibitor 1; fused; mouse; homolog of; axin 1; AXIN; LA16c-314G4.3; MGC52315; axis inhibition protein 1; axis inhibitor 1

axin-1 isoform a; Axin-1; axin-1; axis inhibitor 1; fused, mouse, homolog of; axis inhibition protein 1; protein phosphatase 1, regulatory subunit 49; axin 1; Axis inhibition protein 1; hAxin

AXIN1

AXIN1; AXIN1; AXIN; PPP1R49; AXIN; hAxin

AXIN1_HUMAN

Polyclonal

Goat

Human, Mouse, Rat, Dog, Cow

862

FEEKIIGKVEKVD

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Immunohistochemistry: In paraffin embedded Human Adrenal Gland shows membranous and fibrous staning in and around secretory cells of the zona fasciculata in the cortex. Recommended concentration, 3-5ug/ml. Western Blot: Preliminary experiments gave bands at approx 150kDa and 22-24kDa in Human Brain (Hippocampus) lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 95.6kDa according to NP_003493.1 and 91.7kDa according to NP_851393.1. The detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Peptide ELISA: antibody detection limit dilution 1:128000.

Epitope: C Terminus. Immunogen: Peptide with sequence C-FEEKIIGKVEKVD, from the C Terminus of the protein sequence according to NP_003493.1; NP_851393.1. Corresponding Immunizing Peptide: Catalog #MBS426930

Important Note: This antibody is expected to recognise both reported isoforms.

27501450

NP_003493.1

NM_003502.3

91,653 Da

AMER1 Mutants Destabilize The Destruction Complex Pathway 1016198!!APC Truncation Mutants Are Not K63 Polyubiquitinated Pathway 1016193!!APC Truncation Mutants Have Impaired AXIN Binding Pathway 1016194!!AXIN Missense Mutants Destabilize The Destruction Complex Pathway 1016197!!AXIN Mutants Destabilize The Destruction Complex, Activating WNT Signaling Pathway 1016195!!Basal Cell Carcinoma Pathway 83113!!Basal Cell Carcinoma Pathway 525!!Beta-catenin Phosphorylation Cascade Pathway 106512!!C-MYC Pathway 169344!!Canonical Wnt Signaling Pathway 138032

This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2010]

0.1 mg

290 USD