antibody

Goat anti-Silver homologue/Pmel 17 (C-terminus) Antibody

MBS420909

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat

western blot, enzyme immunoassay

Antibody

Goat anti-Silver homologue/Pmel 17 (C-terminus) Antibody

Silver homologue/Pmel 17

silver homolog (mouse); PMEL17; SILV; D12S53E; ME20; SI; SIL; gp100; Melanocyte protein mel 17; Pmel 17; Silver, mouse, homolog of; silver (mouse homolog) like; silver (mouse homolog)-like; silver homolog; silver homolog (mouse) antibody; PMEL17 antibody; SILV antibody; D12S53E antibody; ME20 antibody; SI antibody; SIL antibody; gp100 antibody; Melanocyte protein mel 17 antibody; Pmel 17 antibody; Silver; mouse; homolog of antibody; silver (mouse homolog) like antibody; silver (mouse homolog)-like antibody; silver homolog antibody; Silver homologue; Pmel 17; Silver homologue / Pmel 17 (C-terminus)

melanocyte protein PMEL isoform 3 preproprotein; Melanocyte protein PMEL; melanocyte protein PMEL; premelanosome protein; ME20-M; ME20M

SILV

PMEL; PMEL; P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E; D12S53E; PMEL17; SILV; ME20M; ME20-S; ME20S

PMEL_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat

661

CPIGENSPLLSGQQ

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 26kDa band observed in Human Skin lysates, corresponding to the M-beta fragment of the precursor protein (Leonhardt et al, Mol Biol Cell.2013 Apr;24(7): 964-81. PMID: 23389629). Calculated MW of 70.3kDa according to NP_008859.1. In transfected HEK293 transiently expressing Human SILV a band of approx.70kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3ug/ml.

Immunogen: Peptide with sequence CPIGENSPLLSGQQ, from the C Terminus of the protein sequence according to NP_008859.1. Epitope: C Terminus

5902084

NP_008859.1

NM_006928.4

66,729 Da

This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]

SILV: Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. Belongs to the PMEL/NMB family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Vesicle. Chromosomal Location of Human Ortholog: 12q13-q14. Cellular Component: endoplasmic reticulum membrane; extracellular region; Golgi apparatus; integral to plasma membrane; melanosome; plasma membrane. Molecular Function: protein binding. Biological Process: melanin biosynthetic process; melanosome organization and biogenesis; pigmentation during development

0.1 mg

300 USD