catalog number :
MBS420892
products full name :
Goat anti-ARNO / cytohesin 2 Antibody
products short name :
ARNO/cytohesin 2
products name syn :
ARNO; cytohesin 2; pleckstrin homology; Sec7 and coiled-coil domains 2 (cytohesin-2); CTS18; CTS18.1; PSCD2L; SEC7L; Sec7p-L; Sec7p-like; ARF exchange factor; ARF nucleotide-binding site opener; pleckstrin homology; Sec7 and coiled-coil domains 2-like; ARNO; cytohesin 2; pleckstrin homology, Sec7 and coiled-coil domains 2 (cytohesin-2); CTS18; CTS18.1; PSCD2L; SEC7L; Sec7p-L; Sec7p-like; ARF exchange factor; ARF nucleotide-binding site opener; pleckstrin homology, Sec7 and coiled-coil domains 2-like
other names :
pleckstrin homology, Sec7 and coiled/coil domains 2 isoform 2; Cytohesin-2; cytohesin-2; CTC-273B12.8; ARF exchange factor; ARF nucleotide-binding site opener; PH, SEC7 and coiled-coil domain-containing protein 2; pleckstrin homology, Sec7 and coiled-coil domains 2 (cytohesin-2); pleckstrin homology, Sec7 and coiled/coil domains 2 (cytohesin-2); cytohesin 2; ARF exchange factor; ARF nucleotide-binding site opener; Protein ARNO; PH, SEC7 and coiled-coil domain-containing protein 2
products gene name :
PSCD2
other gene names :
CYTH2; CYTH2; ARNO; CTS18; PSCD2; SEC7L; PSCD2L; CTS18.1; Sec7p-L; Sec7p-like; ARNO; PSCD2; PSCD2L; Protein ARNO
uniprot entry name :
CYH2_HUMAN
reactivity :
Human, Mouse, Rat, Dog
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx 48kDa band observed in Human Brain (Cerebellum) and Rat Brain lysates (calculated MW of 46.5kDa according to NP_004219.2 and NP_059431.1). Recommended concentration: 0.5-1.5ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000.
other info1 :
Epitope: N Terminus. Immunogen: Peptide with sequence EDGVYEPPDLTP-C, from the N Terminus of the protein sequence according to NP_004219.2; NP_059431.1. Corresponding Immunizing Peptide: Catalog #MBS425983
other info2 :
Important Note: This antibody is expected to recognise both reported isoforms (NP_004219.2; NP_059431.1).
ncbi acc num :
NP_004219.2
ncbi gb acc num :
NM_004228.4
ncbi mol weight :
46,489 Da
ncbi pathways :
Arf1 Pathway 137927!!Arf6 Signaling Events Pathway 138034!!Class I PI3K Signaling Events Pathway 138022!!Endocytosis Pathway 102279!!Endocytosis Pathway 102181
ncbi summary :
The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with ARF1, ARF3, and ARF6 and is 83% homologous to CYTH1. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]