catalog number :
MBS420850
products full name :
Goat anti-HRS / HGS Antibody
products short name :
HRS/HGS
products name syn :
HGS; hepatocyte growth factor-regulated tyrosine kinase substrate; HGNC:4897; HRS; ZFYVE8; human growth factor-regulated tyrosine kinase substrate; HGS; hepatocyte growth factor-regulated tyrosine kinase substrate; HGNC:4897; HRS; ZFYVE8; human growth factor-regulated tyrosine kinase substrate
other names :
hepatocyte growth factor-regulated tyrosine kinase substrate; Hepatocyte growth factor-regulated tyrosine kinase substrate; hepatocyte growth factor-regulated tyrosine kinase substrate; protein pp110; human growth factor-regulated tyrosine kinase substrate; hepatocyte growth factor-regulated tyrosine kinase substrate; Hrs; Protein pp110
other gene names :
HGS; HGS; HRS; HRS
uniprot entry name :
HGS_HUMAN
reactivity :
Human, Mouse, Rat, Dog
sequence :
NRNYWEKKQEEARK
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
app notes :
Immunohistochemistry: In paraffin embedded Human Testis shows strong staining of Leydig cells. Recommended concentration, 1-2ug/ml. Western Blot: Preliminary experiments gave bands at approx 140kDa and 26kDa in Human lysates of monocytic leukemia cell line U937 after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 86.2kDa according to NP_004703.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). Peptide ELISA: antibody detection limit dilution 1:32000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-NRNYWEKKQEEARK, from the internal region of the protein sequence according to NP_004703.1.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS425584
ncbi acc num :
NP_004703.1
ncbi gb acc num :
NM_004712.4
ncbi mol weight :
76,376 Da
ncbi pathways :
CXCR4-mediated Signaling Events Pathway 137910!!Disease Pathway 530764!!EGFR Downregulation Pathway 106342!!ESCRT-0 Complex Pathway 413472!!ESCRT-0 Complex Pathway 890623!!Endocytosis Pathway 102279!!Endocytosis Pathway 102181!!Endosomal Sorting Complex Required For Transport (ESCRT) Pathway 205244!!Internalization Of ErbB1 Pathway 138013!!Membrane Trafficking Pathway 106160
ncbi summary :
The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq, Dec 2010]