antibody

Goat anti-PAI1/SERPINE1 Antibody

MBS420848

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, dog

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-PAI1/SERPINE1 Antibody

PAI1/SERPINE1

Goat Anti-PAI1 / SERPINE1, Biotinylated Antibody; SERPINE1; PAI1; PAI; PAI-1; PLANH1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; plasminogen activator inhibitor, type I; serpin peptidase inhibitor, clade E (nexin, plasminogen activator in; SERPINE1 antibody; PAI1 antibody; PAI antibody; PAI-1 antibody; PLANH1 antibody; serine (or cysteine) proteinase inhibitor; clade E (nexin; plasminogen activator inhibitor type 1); member 1 antibody; plasminogen activator inhibitor; type I antibody; serpin peptidase inhibitor; clade E (nexin; plasminogen activator inhibitor type 1); member 1 antibody; plasminogen activator inhibitor-1 antibody; PAI1 / SERPINE1

plasminogen activator inhibitor 1; Plasminogen activator inhibitor 1; plasminogen activator inhibitor 1; serpin family E member 1; Endothelial plasminogen activator inhibitor; Serpin E1

SERPINE1

PAI1

SERPINE1; SERPINE1; PAI; PAI1; PAI-1; PLANH1; PAI1; PLANH1; PAI; PAI-1

PAI1_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Dog

402

GFKIDDKGMAPALRH

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Placenta shows strong staining of cytotrophoblasts. Recommended concentration, 4-6ug/ml. A customer successfully used this product on Human Breast cancer. Western Blot: Approx 48kDa band observed in Human Liver, Lung and HeLa lysates, while approx. 45kDa observed in lyates of cell lines A549 and HepG2 (calculated MW of 45.1kDa according to NP_000593.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3ug/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant PAI1 captured by a rabbit antibody. Recommended reporter concentration: 1.5-3ug/ml.

Immunogen: Peptide with sequence C-GFKIDDKGMAPALRH, from the internal region of the protein sequence according to NP_000593.1. Epitope: Internal region

10835159

NP_000593.1

NM_000602.4

43,404 Da

AGE-RAGE Signaling Pathway In Diabetic Complications (1319988); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); Adipogenesis Pathway (198832); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (1269872); Blood Clotting Cascade Pathway (198840); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Circadian Clock Pathway (1269871); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073)

This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

SERPINE1: a secreted protein that acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. Belongs to the serpin family. Interacts with VTN. Binds LRP1B; binding is followed by internalization and degradation. Plasma levels of PAI-1 and VCAM-1 together may be useful in predicting post-operative recurrence in patients with colorectal cancer. Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 7q22.1. Cellular Component: extracellular matrix; extracellular region; extracellular space; plasma membrane. Molecular Function: protease binding; protein binding; receptor binding; serine-type endopeptidase inhibitor activity. Biological Process: angiogenesis; chronological cell aging; circadian rhythm; defense response to Gram-negative bacterium; extracellular matrix organization and biogenesis; fibrinolysis; negative regulation of blood coagulation; negative regulation of cell adhesion mediated by integrin; negative regulation of cell migration; negative regulation of fibrinolysis; negative regulation of smooth muscle cell migration; platelet degranulation; positive regulation of angiogenesis; positive regulation of blood coagulation; positive regulation of inflammatory response; positive regulation of interleukin-8 production; positive regulation of receptor-mediated endocytosis; positive regulation of transcription from RNA polymerase II promoter; regulation of receptor activity. Disease: Plasminogen Activator Inhibitor-1 Deficiency

0.1 mg

300 USD