antibody

Goat anti-NOS1 Antibody

MBS420845

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog, cow

western blot, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

Goat anti-NOS1 Antibody

NOS1

NOS1; nitric oxide synthase 1 (neuronal); NOS; neuronal nitric oxide synthase; PnNOS; penile neuronal nitric oxide synthase; penile neuronal NOS; IHPS1; nNOS; nitric oxide synthase 1, neuronal; NOS1 antibody; nitric oxide synthase 1 (neuronal) antibody; NOS antibody; neuronal nitric oxide synthase antibody; PnNOS antibody; penile neuronal nitric oxide synthase antibody; penile neuronal NOS antibody; IHPS1 antibody; nNOS antibody; nitric oxide synthase 1; neuronal antibody

nitric oxide synthase, brain isoform 1; Nitric oxide synthase, brain; nitric oxide synthase, brain; nitric oxide synthase 1; Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOS

NOS1

NOS1; NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS; N-NOS; nNOS

NOS1_HUMAN

Polyclonal

Goat

Tested: Human, Marmoset, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

1434

ESKKDTDEVFSS

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: This antibody has been successfully used in IHC for mouse olfactory bulb as described in the following paper: Fried et al, Cell Tissue Res.2010 Mar;339(3): 463-79; PMID: 20140458. Successful results were also reported by anonymous customers in Rat Cerebral Cortex and in Marmoset Cerebellum. Western Blot: Approx 160-170kDa band observed in Human Skeletal Muscle and Mouse Brain lysates (calculated MW of 161kDa according to NP_000611.1). Recommended concentration: 0.1-0.3ug/ml. Immunofluorescence: This product has been successfully used for IF as reported (PMID: 20140458).

Immunogen: Peptide with sequence C-ESKKDTDEVFSS, from the C Terminus of the protein sequence according to NP_000611.1. Epitope: C Terminus

10835173

NP_000611.1

NM_000620.4

164,779 Da

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Arginine Biosynthesis Pathway (82943); Arginine Biosynthesis Pathway (306)

The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

nNOS: nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described. Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase. Chromosomal Location of Human Ortholog: 12q24.22. Cellular Component: azurophil granule; caveola; cytoplasm; cytoskeleton; cytosol; dendritic spine; lipid raft; mitochondrial outer membrane; mitochondrion; nuclear membrane; perinuclear region of cytoplasm; photoreceptor inner segment; postsynaptic density; protein complex; sarcolemma; sarcoplasmic reticulum; synapse; T-tubule; vesicle membrane; Z disc. Molecular Function: cadmium ion binding; calmodulin binding; enzyme binding; FAD binding; FMN binding; heme binding; iron ion binding; NADP binding; nitric-oxide synthase activity; protein binding; protein homodimerization activity; sodium channel regulator activity. Biological Process: aging; arginine catabolic process; behavioral response to cocaine; cell redox homeostasis; exogenous drug catabolic process; female pregnancy; multicellular organismal response to stress; myoblast fusion; negative regulation of apoptosis; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of heart contraction; negative regulation of hydrolase activity; negative regulation of insulin secretion; negative regulation of potassium ion transport; negative regulation of serotonin uptake; negative regulation of vasoconstriction; neurotransmitter biosynthetic process; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; peptidyl-cysteine S-nitrosylation; positive regulation of guanylate cyclase activity; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of vasodilation; reduction of cytosolic calcium ion concentration; regulation of neurogenesis; regulation of sensory perception of pain; regulation of sodium ion transport; response to activity; response to estrogen stimulus; response to ethanol; response to heat; response to hypoxia; response to lead ion; response to lipopolysaccharide; response to nicotine; response to peptide hormone stimulus; response to vitamin E; striated muscle contraction. Disease: Pyloric Stenosis, Infantile Hypertrophic, 1

0.1 mg

300 USD