antibody

Goat anti-REV1L Antibody

MBS420843

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog, cow

immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-REV1L Antibody

REV1L

REV1; REV1L; REV1-like (yeast); REV1; REV1 protein; REV1 (yeast homolog)- like; REV1 homolog (S. cerevisiae); FLJ21523; MGC163283; MGC26225; REV1- like; REV1 antibody; REV1L antibody; REV1-like (yeast) antibody; REV1 antibody; REV1 protein antibody; REV1 (yeast homolog)- like antibody; REV1 homolog (S. cerevisiae) antibody; FLJ21523 antibody; MGC163283 antibody; MGC26225 antibody; REV1- like antibody

DNA repair protein REV1 isoform 1; DNA repair protein REV1; DNA repair protein REV1; REV1, DNA directed polymerase; Alpha integrin-binding protein 80; AIBP80; Rev1-like terminal deoxycytidyl transferase

REV1

REV1L

REV1; REV1; REV1L; AIBP80; REV1L; AIBP80

REV1_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

1251

VLQQTYGSTLKVT

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 8000. Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining in spermatogonia and spermatocytes. Recommended concentration, 4-6ug/ml. Western Blot: No signal obtained yet but low background observed in MOLT-4 lysates at up to 1.5ug/ml.

Immunogen: Peptide with sequence C-VLQQTYGSTLKVT, from the C Terminus of the protein sequence according to NP_057400.1; NP_001032961.1. Epitope: C Terminus

7706681

NP_057400.1

NM_016316.3

43,649 Da

DNA Damage Bypass Pathway (1270367); DNA Repair Pathway (1270350); Fanconi Anemia Pathway (377262); Fanconi Anemia Pathway (377128); Termination Of Translesion DNA Synthesis Pathway (1270374); Translesion Synthesis By POLI Pathway (1270373); Translesion Synthesis By POLK Pathway (1270372); Translesion Synthesis By REV1 Pathway (1270370); Translesion Synthesis By Y Family DNA Polymerases Bypasses Lesions On DNA Template Pathway (1270369)

This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. [provided by RefSeq, Mar 2016]

REV1L: Deoxycytidyl transferase involved in DNA repair. Transfers a dCMP residue from dCTP to the 3'-end of a DNA primer in a template-dependent reaction. May assist in the first step in the bypass of abasic lesions by the insertion of a nucleotide opposite the lesion. Required for normal induction of mutations by physical and chemical agents. Belongs to the DNA polymerase type-Y family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Transferase; EC 2.7.7.-; DNA repair, damage. Chromosomal Location of Human Ortholog: 2q11.1-q11.2. Cellular Component: nucleoplasm. Molecular Function: damaged DNA binding; deoxycytidyl transferase activity; metal ion binding; protein binding. Biological Process: bypass DNA synthesis; DNA replication; error-prone postreplication DNA repair; response to UV

0.1 mg

300 USD