antibody

Goat anti-PRRX1 Antibody

MBS420837

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse

western blot, enzyme immunoassay

Antibody

Goat anti-PRRX1 Antibody

PRRX1

PRRX1; paired related homeobox 1; PHOX1; PMX1; PRX1; homeobox protein PHOX1; paired mesoderm homeo box 1; paired mesoderm homeobox 1; PRRX1 antibody; paired related homeobox 1 antibody; PHOX1 antibody; PMX1 antibody; PRX1 antibody; homeobox protein PHOX1 antibody; paired mesoderm homeo box 1 antibody; paired mesoderm homeobox 1 antibody

paired mesoderm homeobox protein 1 isoform pmx-1a; Paired mesoderm homeobox protein 1; paired mesoderm homeobox protein 1; paired related homeobox 1; Homeobox protein PHOX1; Paired-related homeobox protein 1; PRX-1

PRRX1

PRRX1; PRRX1; PMX1; PRX1; AGOTC; PHOX1; PRX-1; PMX1; PRX-1

PRRX1_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse

217

GSDTPQQDNDQ

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 28kDa band observed in Human Heart lysates (calculated MW of 27.3kDa according to NP_073207.1). Recommended concentration: 1-3ug/ml.

Immunogen: Peptide with sequence C-GSDTPQQDNDQ, from the internal region of the protein sequence according to NP_008833.1; NP_073207.1. Epitope: Internal region

Note: This antibody is expected to recognise both reported isoforms (NP_008833.1; NP_073207.1).

5902024

NP_008833.1

NM_006902.4

24,396 Da

The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription co-activator, enhancing the DNA-binding activity of serum response factor, a protein required for the induction of genes by growth and differentiation factors. The protein regulates muscle creatine kinase, indicating a role in the establishment of diverse mesodermal muscle types. Alternative splicing yields two isoforms that differ in abundance and expression patterns. [provided by RefSeq, Jul 2008]

PRRX1: Acts as a transcriptional regulator of muscle creatine kinase (MCK) and so has a role in the establishment of diverse mesodermal muscle types. The protein binds to an A/T-rich element in the muscle creatine enhancer. Defects in PRRX1 are the cause of agnathia-otocephaly complex (AGOTC). AGOTC is a rare condition characterized by mandibular hypoplasia or agnathia, ventromedial auricular malposition (melotia) and/or auricular fusion (synotia), and microstomia with oroglossal hypoplasia or aglossia. Holoprosencephaly is the most commonly identified association, but skeletal, genitourinary, and cardiovascular anomalies, and situs inversus have been reported. The disorder is almost always lethal. Belongs to the paired homeobox family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 1q24. Cellular Component: nucleolus; nucleus. Molecular Function: sequence-specific DNA binding; transcription coactivator activity. Biological Process: artery morphogenesis; cartilage development; embryonic cranial skeleton morphogenesis; embryonic limb morphogenesis; inner ear morphogenesis; middle ear morphogenesis; negative regulation of transcription from RNA polymerase II promoter; neuron fate determination; palate development; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; positive regulation of transcription from RNA polymerase II promoter. Disease: Agnathia-otocephaly Complex

0.1 mg

300 USD