antibody

Goat anti-PIWI/HIWI Antibody

MBS420816

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog

immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-PIWI/HIWI Antibody

PIWI/HIWI

PIWIL1; HIWI; PIWI; piwi-like 1 (Drosophila); piwi (Drosophila)-like 1; homolog of Drosophila piwi; MIWI; piwi homolog; piwi-like 1; PIWIL1 antibody; HIWI antibody; PIWI antibody; piwi-like 1 (Drosophila) antibody; piwi (Drosophila)-like 1 antibody; homolog of Drosophila piwi antibody; MIWI antibody; piwi homolog; piwi-like 1 antibody; PIWI / HIWI

piwi-like protein 1 isoform 1; Piwi-like protein 1; piwi-like protein 1; piwi like RNA-mediated gene silencing 1

PIWIL1

PIWI; HIWI

PIWIL1; PIWIL1; HIWI; MIWI; PIWI; CT80.1; HIWI

PIWL1_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

861

SNRKDKYDAIKKY

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 64000. Immunohistochemistry: In paraffin embedded Human Testis shows textured cytoplasm staining in all germ cells. Recommended concentration, 4-6ug/ml. Western Blot: Preliminary experiments showed a 100kDa band but also bands at approx 35kDa and 26-28kDa in Human Ovary, Placenta, Prostate and Testis lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 98.6kDa according to NP_004755.2. All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).

Immunogen: Peptide with sequence C-SNRKDKYDAIKKY, from the internal region of the protein sequence according to NP_004755.2; NP_001177900.1. Epitope: Internal region

Note: This antibody is expected to recognise both reported isoforms (NP_004755.2; NP_001177900.1).

66346725

NP_004755.2

NM_004764.4

89,484 Da

Dorso-ventral Axis Formation Pathway (114227); Dorso-ventral Axis Formation Pathway (472); Gene Expression Pathway (1269649); Gene Silencing By RNA Pathway (1269719); PIWI-interacting RNA (piRNA) Biogenesis Pathway (1269724)

This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

PIWIL1: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer- independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Isoform 3 may be a negative developmental regulator. Interacts (via Piwi domain) with DICER1, suggesting that it forms ribonucleoprotein RISC complexes. This interaction is regulated by HSP90AB1 activity. Interacts with MAEL, KIF17, PABPC1, PRMT5 and WDR77. Interacts (when methylated on arginine residues) with TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9. Isoform 3 is down-regulated in CD34(+) hematopoietic cells during differentiation. Detected in most fetal and adult tissues. Expressed in testes, specifically in germline cells; detected in spermatocytes and spermatids during spermatogenesis. Increased expression in testicular tumors originating from embryonic germ cells with retention of germ cells phenotype. No expression in testicular tumors of somatic origin, such as Sertoli cell and Leydig cell tumors. Overexpressed in gastric cancer cells. Isoform 3 is ubiquitously expressed, and specifically in CD34+ hematopoietic progenitor cells but not in more differentiated cells. Belongs to the argonaute family. Piwi subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, peripheral. Chromosomal Location of Human Ortholog: 12q24.33. Cellular Component: cytoplasm; mRNA cap complex; nucleus; polysome. Molecular Function: mRNA binding; protein binding; protein kinase binding; single-stranded RNA binding. Biological Process: meiotic cell cycle; multicellular organismal development; regulation of translation; RNA-mediated gene silencing; spermatid development

0.1 mg

300 USD