antibody

Goat anti-MYD88 Antibody

MBS420798

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-MYD88 Antibody

MYD88

Goat Anti-MYD88, Biotinylated Antibody; MYD88; myeloid differentiation primary response gene (88); MYD88D; myeloid differentiation primary response gene 88; MYD88 antibody; myeloid differentiation primary response gene (88) antibody; MYD88D antibody; myeloid differentiation primary response gene 88 antibody

myeloid differentiation primary response protein MyD88 isoform 1; Myeloid differentiation primary response protein MyD88; myeloid differentiation primary response protein MyD88; myeloid differentiation primary response 88

MYD88

MYD88; MYD88; MYD88D

MYD88_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog

317

IKYKAMKKEFP

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: In paraffin embedded Human Tonsil shows staining of clusters of cells some of which are likely plasma cells. Recommended concentration, 4-6ug/ml. Western Blot: Approx 33kDa band observed in HumanThymus lysates (calculated MW of 33.2kDa according to NP_002459.1). Recommended concentration: 0.1-0.3ug/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant MYD88 captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml.

Immunogen: Peptide with sequence C-IKYKAMKKEFP, from the internal region of the protein sequence according to NP_001166038.1; NP_002459.2; NP_001166039.1. Epitope: Internal region

Note: This antibody is expected to recognize reported isoforms 1, 2 and 3 (NP_001166038.1; NP_002459.2; NP_001166039.1).

289546503

NP_001166038.1

NM_001172567.1

34,097 Da

AGE/RAGE Pathway (698754); Activated TLR4 Signalling Pathway (1269236); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Apoptosis Modulation And Signaling Pathway (198822); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (1269310); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1269268); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (1269276)

This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

MYD88: Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1, IRAK2 and IRAK4 via their respective TIR domains. Interacts with IL18R1. Interacts with BMX, IL1RL1 and IRF7. Interacts with LRRFIP1 and LRRFIP2; this interaction positively regulates Toll-like receptor (TLR) signaling in response to agonist. Interacts with FLII. LRRFIP1 and LRRFIP2 compete with FLII for MYD88-binding. Interacts with IRF1. Ubiquitous. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 3p22. Cellular Component: cytosol; endosome membrane; plasma membrane. Molecular Function: death receptor binding; identical protein binding; protein binding. Biological Process: cell surface receptor linked signal transduction; defense response to Gram-positive bacterium; inflammatory response; innate immune response; MyD88-dependent toll-like receptor signaling pathway; negative regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of interleukin-17 production; positive regulation of interleukin-23 production; positive regulation of interleukin-6 production; regulation of inflammatory response; signal transduction; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway. Disease: Macroglobulinemia, Waldenstrom, Susceptibility To, 1; Myd88 Deficiency

0.1 mg

300 USD