antibody

Goat anti-PNKP/PNK Antibody

MBS420796

0.1 mg

300 USD

polyclonal

goat

nonconjugated

western blot, enzyme immunoassay

Antibody

Goat anti-PNKP/PNK Antibody

PNKP/PNK

PNKP; polynucleotide kinase 3'-phosphatase; PNK; polynucleotide kinase 3-prime-phosphatase; Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP); PNKP antibody; polynucleotide kinase 3 1-phosphatase antibody; PNK antibody; polynucleotide kinase 3-prime-phosphatase antibody; Homo sapiens polynucleotide kinase 3 1-phosphatase (PNKP) antibody; PNKP / PNK

bifunctional polynucleotide phosphatase/kinase; Bifunctional polynucleotide phosphatase/kinase; bifunctional polynucleotide phosphatase/kinase; polynucleotide kinase 3'-phosphatase; DNA 5'-kinase/3'-phosphatase; Polynucleotide kinase-3'-phosphataseIncluding the following 2 domains:Polynucleotide 3'-phosphatase (EC:3.1.3.32)Alternative name(s):2'(3')-polynucleotidase

PNKP

PNK

PNKP; PNKP; PNK; AOA4; MCSZ; EIEE10

PNKP_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human

521

EPRLGRLYCQFSEG

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Sucessfully used to detect recombinant human PNKP (1/1000 dilution).

Immunogen: Peptide with sequence EPRLGRLYCQFSEG, from the C Terminus of the protein sequence according to NP_009185.2. Epitope: C Terminus

31543419

NP_009185.2

NM_007254.3

53,004 Da

APEX1-Independent Resolution Of AP Sites Via The Single Nucleotide Replacement Pathway (1270363); Base Excision Repair Pathway (1270351); DNA Repair Pathway (1270350); Resolution Of Abasic Sites (AP Sites) Pathway (1270359)

This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.[provided by RefSeq, Sep 2010]

Pnk1: polynucleotide kinase 3'-phosphatase. May be involved in double-strand break repair by non-homologous end joining. Required for normal response to DNA damage by gamma-radiation or camptothecin in S. pombe. Protein type: Phosphatase (non-protein); EC 2.7.1.78; DNA-binding; EC 3.1.3.32; Kinase, other. Chromosomal Location of Human Ortholog: 19q13.3-q13.4. Cellular Component: membrane; nucleolus; nucleoplasm; nucleus. Molecular Function: ATP binding; ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; damaged DNA binding; double-stranded DNA binding; endonuclease activity; nucleotide kinase activity; polynucleotide 3'-phosphatase activity; protein binding; purine nucleotide binding. Biological Process: dephosphorylation; DNA damage response, detection of DNA damage; DNA repair; DNA-dependent DNA replication; nucleotide phosphorylation; nucleotide-excision repair, DNA damage removal; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; response to oxidative stress; response to radiation. Disease: Ataxia-oculomotor Apraxia 4; Microcephaly, Seizures, And Developmental Delay

0.1 mg

300 USD