antibody

Goat anti-FOXL2/BPES Antibody

MBS420788

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, cow

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-FOXL2/BPES Antibody

FOXL2/BPES

FOXL2; BPES; forkhead box L2; PFRK; BPES1; PINTO; forkhead transcription factor FOXL2; blepharophimosis, epicanthus inversus and ptosis; Blepharophimosis, epicanthus inversus, and ptosis 1; POF3; FOXL2 antibody; BPES antibody; forkhead box L2 antibody; PFRK antibody; BPES1 antibody; PINTO antibody; forkhead transcription factor FOXL2 antibody; blepharophimosis; epicanthus inversus and ptosis antibody; Blepharophimosis; epicanthus inversus; and ptosis 1 antibody; POF3 antibody; FOXL2 / BPES

forkhead box protein L2; Forkhead box protein L2; forkhead box protein L2; forkhead box L2

FOXL2

BPES

FOXL2; FOXL2; BPES; PFRK; POF3; BPES1; PINTO

FOXL2_HUMAN

Polyclonal

Goat

Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Cow

376

DSKTGALHSRLDL

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Ovary shows nuclear staining in select cells. Recommended concentration, 2-4ug/ml. Western Blot: Approx 50kDa band observed in Human and Mouse Ovary lysates (calculated MW of 38.8kDa according to Human NP_075555.1 and 38.9kDa according to Mouse NP_036150.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration 0.3-1ug/ul.

Immunogen: Peptide with sequence C-DSKTGALHSRLDL, from the C Terminus of the protein sequence according to NP_075555.1. Epitope: C Terminus

Blount AL, Schmidt K, Justice NJ, Vale WW, Fischer WH, Bilezikjian LM.; FoxL2 and Smad3 coordinately regulate follistatin gene transcription.; J Biol Chem.2009 Mar 20;284(12): 7631-45. Epub 2008 Dec 23.; Pubmed 19106105

12751477

NP_075555.1

NM_023067.3

38,772 Da

This gene encodes a forkhead transcription factor. The protein contains a fork-head DNA-binding domain and may play a role in ovarian development and function. Mutations in this gene are a cause of blepharophimosis syndrome and premature ovarian failure 3. [provided by RefSeq, Jun 2009]

FOXL2: Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans- differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9. Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen. Is a regulator of CYP19 expression. Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element. Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2. Defects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES); also known as blepharophimosis syndrome. It is an autosomal dominant disorder characterized by eyelid dysplasia, small palpebral fissures, drooping eyelids and a skin fold running inward and upward from the lower lid. In type I BPSE (BPES1) eyelid abnormalities are associated with female infertility. Affected females show an ovarian deficit due to primary amenorrhea or to premature ovarian failure (POF). In type II BPSE (BPES2) affected individuals show only the eyelid defects. There is a mutational hotspot in the region coding for the poly-Ala domain, since 30% of all mutations in the ORF lead to poly-Ala expansions, resulting mainly in BPES type II. Defects in FOXL2 are a cause of premature ovarian failure type 3 (POF3). An ovarian disorder defined as the cessation of ovarian function under the age of 40 years. It is characterized by oligomenorrhea or amenorrhea, in the presence of elevated levels of serum gonadotropins and low estradiol. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 3q23. Cellular Component: nucleus. Molecular Function: caspase regulator activity; DNA binding; estrogen receptor binding; protein binding; transcription factor activity; ubiquitin conjugating enzyme binding. Biological Process: cell differentiation; DNA fragmentation during apoptosis; embryonic eye morphogenesis; extraocular skeletal muscle development; female somatic sex determination; granulosa cell differentiation; menstruation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; oocyte growth; ovarian follicle development; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of follicle-stimulating hormone secretion; positive regulation of luteinizing hormone secretion; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; single fertilization; transcription from RNA polymerase II promoter; uterus development. Disease: Blepharophimosis, Ptosis, And Epicanthus Inversus; Premature Ovarian Failure 3

0.1 mg

300 USD