antibody

Goat anti-OCT4 / POU5F1 Antibody

MBS420786

0.1 mg

290 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog

western blot, enzyme immunoassay

Antibody

Goat anti-OCT4 / POU5F1 Antibody

OCT4/POU5F1

POU5F1; OTF4; POU domain; class 5; transcription factor 1; HGNC:9221; MGC22487; OCT3; OTF3; POU-type homeodomain-containing DNA-binding protein; octamer-binding transcription factor-3; POU class 5 homeobox 1; DADB-104B20.2; OCT4; POU domain; class 5; transcription factor 1; POU5F1; OTF4; POU domain, class 5, transcription factor 1; HGNC:9221; MGC22487; OCT3; OTF3; POU-type homeodomain-containing DNA-binding protein; octamer-binding transcription factor-3; POU class 5 homeobox 1; DADB-104B20.2; OCT4; POU domain, class 5, transcription factor 1

POU domain, class 5, transcription factor 1 isoform 1; POU domain, class 5, transcription factor 1; POU domain, class 5, transcription factor 1; octamer-binding protein 3; octamer-binding protein 4; POU domain transcription factor OCT4; octamer-binding transcription factor 3; POU-type homeodomain-containing DNA-binding protein; POU class 5 homeobox 1; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3

POU5F1

POU5F1; POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4; OCT3; OCT4; OTF3; Oct-3; Oct-4; OTF-3

PO5F1_HUMAN

Polyclonal

Goat

Human, Mouse, Rat, Dog

360

VTTLGSPMHSN

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Western Blot: Approx 30kDa band observed in Human Liver and Placenta lysates (calculated MW of 30.1kDa according to NP_976034.3). Recommended concentration: 1-3ug/ml. Peptide ELISA: antibody detection limit dilution 1:2000.

Epitope: C Terminus. Immunogen: Peptide with sequence C-VTTLGSPMHSN, from the C Terminus of the protein sequence according to NP_002692.2; NP_976034.3. Corresponding Immunizing Peptide: Catalog #MBS425947

Important Note: This antibody is expected to recognise both reported isoforms (NP_002692.2 and NP_976034.3)

42560248

NP_002692.2

NM_002701.5

30,085 Da

Cardiac Progenitor Differentiation Pathway 712094!!Developmental Biology Pathway 477129!!HIF-2-alpha Transcription Factor Network Pathway 137956!!POU5F1 (OCT4), SOX2, NANOG Activate Genes Related To Proliferation Pathway 980456!!POU5F1 (OCT4), SOX2, NANOG Repress Genes Related To Differentiation Pathway 980457!!SIDS Susceptibility Pathways 198901!!Signaling Pathways Regulating Pluripotency Of Stem Cells 1026136!!Signaling Pathways Regulating Pluripotency Of Stem Cells 1033502!!Transcriptional Regulation Of Pluripotent Stem Cells Pathway 980455!!Wnt Signaling Pathway And Pluripotency 198847

This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]

0.1 mg

290 USD