catalog number :
MBS420772
products full name :
Goat anti-EMI2 Antibody
products short name :
EMI2
products name syn :
FBXO43; EMI2; F-box protein 43; Fbx43; early mitotic inhibitor 2; ERP1; FBXO43; EMI2; F-box protein 43; Fbx43; early mitotic inhibitor 2; ERP1
other names :
F-box only protein 43; F-box only protein 43; F-box only protein 43; early mitotic inhibitor 2; endogenous meiotic inhibitor 2; F-box protein 43; Endogenous meiotic inhibitor 2
products gene name :
FBXO43
other gene names :
FBXO43; FBXO43; EMI2; ERP1; FBX43; EMI2
uniprot entry name :
FBX43_HUMAN
reactivity :
Human, Mouse, Rat, Dog, Pig, Cow
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
app notes :
Western Blot: This product has been successfully used on Rat Pachytene Spermatocytes showing a 71kDa band (Perrard et al, PLoS One. 2009 Oct 5;4(10):e7237. PMID: 19802389) and in Mouse Oocytes (Bernhardt et al, Biol Reprod. 2012 Apr 19;86(4):114. PMID: 22302686). Recommended concentration: 1-3ug/ml. Peptide ELISA: antibody detection limit dilution 1:16000. This product has been successfully used in Immunofluorescence and Flow Cytometry on Rat meiotic Testis cells: (Perrard et al, PLoS One. 2009 Oct 5;4(10):e7237. PMID: 19802389). Recommended concentration: 5-10ug/ml.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence C-PGSAQSKRNLKRL, from the C Terminus of the protein sequence according to NP_001025031.2.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS426665
ncbi acc num :
NP_001025031.2
ncbi gb acc num :
NM_001029860.3
ncbi mol weight :
78,402 Da
ncbi pathways :
Oocyte Meiosis Pathway 126909!!Oocyte Meiosis Pathway 126872
ncbi summary :
Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]