antibody

Goat anti-WNT4 Antibody

MBS420768

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat

western blot, enzyme immunoassay

Antibody

Goat anti-WNT4 Antibody

WNT4

WNT4; wingless-type MMTV integration site family, member 4; WNT-4; WNT-4 protein precursor; RP1-224A6.7; SERKAL; WNT-4 protein; WNT4 antibody; wingless-type MMTV integration site family; member 4 antibody; WNT-4 antibody; WNT-4 protein precursor antibody; RP1-224A6.7 antibody; SERKAL antibody; WNT-4 protein antibody

protein Wnt-4; Protein Wnt-4; protein Wnt-4; Wnt family member 4

WNT4

WNT4; WNT4; WNT-4; SERKAL

WNT4_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat

351

SNWLYLAKLSSVGS

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 40+35kDa band observed in lysates of cell line JURKAT (calculated MW of 39.1kDa according to NP_110388.2). Recommended concentration: 1-3. 0ug/ml.

Immunogen: Peptide with sequence C-SNWLYLAKLSSVGS, from the internal region of the protein sequence according to NP_110388.2. Epitope: Internal region

17402922

NP_110388.2

NM_030761.4

32,954 Da

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Beta-catenin Independent WNT Signaling Pathway (1269610); Class B/2 (Secretin Family Receptors) Pathway (1269570); DNA Damage Response (only ATM Dependent) Pathway (198827); GPCR Ligand Binding Pathway (1269544); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889)

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and is the first signaling molecule shown to influence the sex-determination cascade. It encodes a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. This gene and a nuclear receptor known to antagonize the testis-determining factor play a concerted role in both the control of female development and the prevention of testes formation. This gene and another two family members, WNT2 and WNT7B, may be associated with abnormal proliferation in breast tissue. Mutations in this gene can result in Rokitansky-Kuster-Hauser syndrome and in SERKAL syndrome. [provided by RefSeq, Jul 2008]

WNT4: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Overexpression may be associated with abnormal proliferation in human breast tissue. Defects in WNT4 are a cause of Rokitansky-Kuster-Hauser syndrome (RKH syndrome); also called Mayer- Rokitansky-Kuster-Hauser syndrome (MRKH syndrome or MRKH anomaly). RKH syndrome is characterized by utero-vaginal atresia in otherwise phenotypically normal female with a normal 46,XX karyotype. Anomalies of the genital tract range from upper vaginal atresia to total Muellerian agenesis with urinary tract abnormalities. It has an incidence of approximately 1 in 5'000 newborn girls. Defects in WNT4 are the cause of female sex reversal with dysgenesis of kidneys, adrenals, and lungs (SERKAL); also known as SERKAL syndrome. Defects in WNT4 are the cause of Muellerian aplasia (MULLAPL). Belongs to the Wnt family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1p36.23-p35.1. Cellular Component: cell surface; cytoplasm; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane; proteinaceous extracellular matrix. Molecular Function: frizzled binding; receptor agonist activity; transcription corepressor activity. Biological Process: adrenal gland development; androgen biosynthetic process; cell fate commitment; cellular response to starvation; embryonic epithelial tube formation; epithelial to mesenchymal transition; female gonad development; female sex determination; immature T cell proliferation in the thymus; kidney development; liver development; male gonad development; negative regulation of cell differentiation; negative regulation of cell migration; negative regulation of fibroblast growth factor receptor signaling pathway; negative regulation of transcription, DNA-dependent; neuron differentiation; oocyte development; positive regulation of aldosterone biosynthetic process; positive regulation of bone mineralization; positive regulation of collagen biosynthetic process; positive regulation of focal adhesion formation; positive regulation of GTPase activity; positive regulation of meiosis; positive regulation of osteoblast differentiation; positive regulation of stress fiber formation; positive regulation of transcription, DNA-dependent; regulation of cell-cell adhesion; smooth muscle cell differentiation; somatotropin secreting cell differentiation; thyroid stimulating hormone secreting cell differentiation; ureteric bud branching; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin. Disease: 46,xx Sex Reversal With Dysgenesis Of Kidneys, Adrenals, And Lungs; Mullerian Aplasia And Hyperandrogenism

0.1 mg

300 USD