Goat anti-GFAP Antibody | MyBioSource MBS420746 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-GFAP Antibody

catalog :

MBS420746

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog

application :

western blot, ELISA, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420746

products type :

Antibody

products full name :

Goat anti-GFAP Antibody

products short name :

GFAP

products name syn :

GFAP; glial fibrillary acidic protein; FLJ45472; intermediate filament protein; GFAP antibody; glial fibrillary acidic protein antibody; FLJ45472 antibody; intermediate filament protein antibody

other names :

glial fibrillary acidic protein isoform 1; Glial fibrillary acidic protein; glial fibrillary acidic protein; glial fibrillary acidic protein

products gene name :

GFAP

other gene names :

GFAP; GFAP; ALXDRD; GFAP

uniprot entry name :

GFAP_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog

sequence length :

432

sequence :

DGEVIKESKQEHKD

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Cerebellum shows specific staining of astrocytes. Recommended concentration, 2-4ug/ml. Frozen sections of Human Hypothalamus shows staining of astroglial cells. Recommended concentration, 0.01-0. 03ug/ml. Both data obtained from a previous batch (different goat). Western Blot: Approx 48kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates (calculated MW of 49.9kDa according to Mouse NP_034407.2 and Human NP_002046.1). Recommended concentration: 0.01-0.03ug/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant GFAP captured by a rabbit antibody. Recommended reporter concentration: 5-10ug/ml. Please note this data was obtained using a previous batch (different goat).

other info1 :

Immunogen: Peptide with sequence C-DGEVIKESKQEHKD, from the C Terminus of the protein sequence according to NP_002046.1. Epitope: C Terminus

other info2 :

Note: GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.

ncbi gi num :

4503979

ncbi acc num :

NP_002046.1

ncbi gb acc num :

NM_002055.4

ncbi mol weight :

49,508 Da

ncbi pathways :

Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Neural Crest Differentiation Pathway (672460); Nuclear Signaling By ERBB4 Pathway (1269499); Signal Transduction Pathway (1269379); Signaling By ERBB4 Pathway (1269496); Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

uniprot summary :

GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: cytoplasm; cytosol; intermediate filament; intermediate filament cytoskeleton; lysosome; membrane; myelin sheath. Molecular Function: glycoprotein binding; integrin binding; kinase binding; protein binding; structural constituent of cytoskeleton. Biological Process: astrocyte development; Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; intermediate filament organization; neurite regeneration; regulation of neurotransmitter uptake; regulation of protein complex assembly; response to wounding. Disease: Alexander Disease

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage