Goat anti-SART1 Antibody | MyBioSource MBS420712 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-SART1 Antibody

catalog :

MBS420712

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS420712

products type :

Antibody

products full name :

Goat anti-SART1 Antibody

products short name :

SART1

products name syn :

SART1; squamous cell carcinoma antigen recognised by T cells; ARA1; Ara1; HOMS1; MGC2038; SART1259; Snu66; IgE autoantigen; SART1(259) protein; SART1(800) protein; U4/U6. U5 tri-snRNP-associated 110 kDa protein; squamous cell carcinoma antigen recognised b; SART1 antibody; squamous cell carcinoma antigen recognised by T cells antibody; ARA1 antibody; Ara1 antibody; HOMS1 antibody; MGC2038 antibody; SART1259 antibody; Snu66 antibody; IgE autoantigen antibody; SART1(259) protein antibody; SART1(800) protein antibody; U4/U6. U5 tri-snRNP-associated 110 kDa protein antibody; squamous cell carcinoma antigen recognised by T cells antibody; squamous cell carcinoma antigen recognized by T cells 1 antibody; SNRNP110 antibody; small nuclear ribonucleoprotein 110kDa (U4/U6. U5) antibody

other names :

U4/U6.U5 tri-snRNP-associated protein 1; U4/U6.U5 tri-snRNP-associated protein 1; U4/U6.U5 tri-snRNP-associated protein 1; squamous cell carcinoma antigen recognized by T-cells 1; SNU66 homolog; hSnu66; Squamous cell carcinoma antigen recognized by T-cells 1; SART-1; hSART-1; U4/U6.U5 tri-snRNP-associated 110 kDa protein; Allergen: Hom s 1

products gene name :

SART1

other gene names :

SART1; SART1; Ara1; HOMS1; Snu66; SART1259; SNRNP110; hSnu66; SART-1; hSART-1

uniprot entry name :

SNUT1_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow

sequence length :

800

sequence :

GSSKKHRGEKEAA

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 125kDa band observed in HeLa lysates (consistent with Vertegaal et al, below, calculated MW of 90.3 kDa according to NP_005137). Recommended concentration: 0. 03-0.1ug/ml.

other info1 :

Immunogen: Peptide with sequence GSSKKHRGEKEAA-C, from the N Terminus of the protein sequence according to NP_005137.1. Epitope: N Terminus

other info2 :

Note: This antibody is expected to recognise both the human SART1(800) and SART1(259) proteins.

ncbi gi num :

10863889

ncbi acc num :

NP_005137.1

ncbi gb acc num :

NM_005146.4

ncbi mol weight :

90,255 Da

ncbi pathways :

Gene Expression Pathway (1269649); Processing Of Capped Intron-Containing Pre-mRNA Pathway (1269688); Spliceosome Pathway (125136); Spliceosome Pathway (124832); Spliceosome, U4/U6.U5 Tri-snRNP Pathway (413445); Spliceosome, U4/U6.U5 Tri-snRNP Pathway (890601); MRNA Splicing Pathway (1269689); MRNA Splicing - Major Pathway (1269690)

ncbi summary :

This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway. [provided by RefSeq, Jul 2008]

uniprot summary :

SART1: May play a role in mRNA splicing. May also bind to DNA. Belongs to the SNU66/SART1 family. 1 isoforms of the human protein are produced by alternative splicing. Protein type: RNA-binding; Apoptosis; Spliceosome; RNA processing; RNA splicing. Chromosomal Location of Human Ortholog: 11q13.1. Cellular Component: Cajal body; cytoplasm; cytosol; Golgi apparatus; nucleoplasm; U4/U6 x U5 tri-snRNP complex. Molecular Function: protein binding. Biological Process: cell cycle arrest; maturation of 5S rRNA; nuclear mRNA cis splicing, via U2-type spliceosome; nuclear mRNA splicing, via spliceosome; positive regulation of cytotoxic T cell differentiation; spliceosomal snRNP biogenesis

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage